Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

LC3B (human), (recombinant) (GST-tag)

 
BML-UW1155-0500 500 µg 187.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Microtubule-associated proteins 1A/1B light chain 3B
 
MW:~42.1kDa
 
Source:Produced in E. coli.
 
UniProt ID:Q9GZQ8
 
Formulation:Liquid. In PBS.
 
Purity:≥95% (SDS-PAGE)
 
Application Notes:For use in general and selective autophagy studies.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 12 months after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:Autophagy is a highly conserved bulk protein degradation pathway responsible for the turnover of long-lived proteins, disposal of damaged organelles, and clearance of aggregate-prone proteins. Autophagy is involved in various physiological or pathological processes, such as development, host defense response, cancer and neuronal degenerative diseases. It involves concerted action of more than 20 specific autophagy (ATG) proteins that mediate the formation of a double-membrane vesicle, the autophagosome, which engulfs the substrate and delivers it to the lysosome for degradation.

Formation and expansion of the pre-autophagosomal structure in yeast requires the attachment of the ubiquitin-like protein ATG8 via its C-terminal glycine to the amino group of phosphatidylethanolamine (PE), enabling its anchoring to the isolation membrane of the autophagosome. In mammals, ATG8 is represented by at least seven related proteins that fall into two subgroups, LC3- and GABARAP-like proteins. The free and PE linked versions of these proteins are often referred to as LC3-I and LC3-II respectively.
 

General Literature References

Fighting disease by selective autophagy of aggregate-prone proteins: H. Knaevelsrud & A. Simonsen; FEBS Lett. 584, 2635 (2010), Abstract;
Role of autophagy in suppression of inflammation and cancer: E. White, et al. ; Curr. Opin. Cell. Biol. 22, 212 (2010), Abstract;
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol.Cell. 34, 259 (2009), Abstract;
Autophagy as an emerging dimension to adaptive and innate immunity: S. Hussey, et al.; Semin. Immunol. 21, 233 (2009), Abstract;
The emerging role of autophagy in Parkinson's disease: Z.H. Cheung, et al.; Mol. Brain Res. 2, 29 (2009), Abstract;
Autophagy fights disease through cellular self-digestion: M. Mizushima, et al.; Nature 451, 1069 (2008), Abstract;
Autophagosome formation: core machinery and adaptations: Z. Xie, et al.; Nat. Cell. Biol. 9, 1102 (2007), Abstract;
Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B: H. He, et al.; J. Biol. Chem 278, 29278 (2003), Abstract;
Autophagy in the eukaryotic cell: F. Reggiori, et al.; Eukaryot. Cell. 1, 11 (2002), Abstract;
Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein: Y. Xin, et al.; Genomics 744, 408 (2001), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
LC3
By biological activity:
LC3 Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

Brochures
Epigenetics
Epigenetics
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,