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A20 (catalytic domain) (human), (recombinant) (untagged)

 
BML-UW1015-0100 100 µg 258.00 USD
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Product Specification

Alternative Name:Tumor necrosis factor alpha-induced protein 3
, TNAP3, TNFAIP3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20
 
MW:~43kDa
 
Source:Produced in E. coli.
 
UniProt ID:P21580
 
Formulation:Liquid. In 25mM TRIS-HCl, pH 8.5, containing 200mM sodium chloride, 1mM EDTA and 5mM DTT.
 
Purity:≥80% (SDS-PAGE)
 
Application Notes:For use in deubiquitinylation assays.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 1 year after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:The NF-κB (nuclear factor κB) regulator A20 antagonizes IKK [IκB (inhibitor of κB) kinase] activation by modulating Lys63-linked polyubiquitinylation of cytokine-receptor-associated factors including TRAF2/6, RIP1 and the IKK subunit NEMO.

A20 possess both deubiquitinylating and ubiquitin E3 ligase activities, through its N-terminal OTU (ovarian tumour) and seven A20-like ZF (zinc finger) domains respectively and acts by disassembling Lys63-linked polyubiquitin chains from TRAF2/6 and RIP1, subsequently replacing them with Lys48-linked polyubiquitin.

This dual action not only antagonizes downstream signalling, by indirectly stabilizing IκB, but also targets the substrates for proteasomal degradation.
 
Technical Info/Product Notes:The isolated A20 OTU catalytic domain (CD) has been shown to exhibit a preference for Lys48-linked tetraubiquitin in vitro suggesting that additional specificity factors might be required for the physiological function of A20 in cells.

Note: A20CD must be pre-incubated with 10mM DTT at room temperature for 15 minutes to activate the enzyme prior to running activity assays.
 

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