Bromoethyl ubiquitin, (HA-tag) - Enzo Life Sciences

BML-UW0890

Revised 05-Mar-10

Bromoethyl ubiquitin, (HA-tag)
SYNONYMS	HA-Ub-Br
PRODUCT LINE	Ubiquitin & Ubl Signalling
PRODUCT CATEGORY	Ubiquitin Deconjugation Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
BML-UW0890-0025		25 µg	200.00 USD

Product Specification

MW:	~10’266Da
SOURCE/HOST:	Synthetic.
PURITY:	≥95% (HPLC)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with aqueous buffer up to 5mg/ml (~500μM).
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-80°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -80°C.
HANDLING:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots, store at -80°C and use within 1 month.

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General Information

The ubiquitin cascade in its many forms includes a large family of cysteine proteases known as deubiquitinylating enzymes (DUBs). Many members are assigned to this enzyme class by sequence similarity but with little evidence for specific biological activity. HA-tagged ubiquitin molecules derivatised by chemical ligation at the C-terminus with thiol-reactive functional groups can be used as potent, irreversible inhibitors of DUBs through their covalent modification of the active site and, consequently, can act as specific probes for enzymes with DUB activity. Such probes have been utilized for the identification of DUBs and associated components from cell lysates by proteomic methods, as well as rapid demonstration of enzymatic activity for gene products whose functions were inferred from primary structure. The nature of the electrophilic group used as the C-terminal derivative, for example vinyl sulfone (-VS), vinyl methylester (-VME), 2-bromoethyl (-Br) or 2-chloroethyl (-Cl), may determine the subset of DUB enzymes detected. The HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein, facilitates sensitive identification or purification of HA-Ub-Br modified DUBs through recognition by HA-reactive antibodies in free or agarose-immobilised.
BACKGROUND/TECHNICAL INFORMATION
Suggested uses: 1. Active site-directed probes for detection of DUBs. 2. Isolation and identification of DUBs. 3. Identification of DUBs within biological samples. UniProt link P62988: Ubiquitin (human)

General Literature References

A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14: A. Borodovsky, et al.; EMBO J. 20, 5187 (2001) Abstract; Full Text
Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family: A. Borodovsky, et al.; Chem. Biol. 9, 1149 (2002) Abstract
Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells: H. Ovaa, et al.; PNAS 101, 2253 (2004) Abstract; Full Text
Derivitization of the C-terminus of ubiquitin and ubiquitin-like proteins using intein chemistry: methods and uses: K.D. Wilkinson, et al.; Methods Enzymol. 399, 37 (2005) Abstract
Structural basis and specificity of human otubain 1-mediated deubiquitination: M.J. Edelmann, et al.; Biochem. J. 418, 379 (2009) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins