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Linear polyubiquitin chains, sampler pack

 
BML-UW0825-0001 1 Pack 366.00 USD
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Sampler pack for use in deubiquitinylating enzyme assays and linear polyubiquitin binding studies.
bml-uw0825
SDS-PAGE comparison of linear polyubiquitin chains (2µg/lane).
Lane 1: Ub2 (BML-UW0775)
Lane 2: Ub3 (BML-UW0780)
Lane 3: Ub4 (BML-UW0785)
Lane 4: Ub5 (BML-UW0790)
Lane 5: Ub6 (BML-UW0795)
Lane 6: Ub7 (BML-UW0800)
Lane 7: Ub8 (BML-UW0805)
Lane 8: Ub9 (BML-UW0810)
Lane 9: Ub10 (BML-UW0815)
Lane 10: Ub11 (BML-UW0820)
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bml-uw0825

Product Specification

Application Notes:For use in deubiquitinylating enzyme assays and linear polyubiquitin binding studies.
 
Purity:≥90% (SDS-PAGE)
 
Kit/Set Contains:

Name Prod. No. Amount Mol. Weight
Di-ubiquitin (Ub2) (linear) BML-UW0775 25µg 17.1kDa
Tri-ubiquitin (Ub3) (linear) BML-UW0780 25µg 25.7kDa
Tetra-ubiquitin (Ub4) (linear) BML-UW0785 25µg 34.2kDa
Penta-ubiquitin (Ub5) (linear) BML-UW0790 25µg 42.8kDa
Hexa-ubiquitin (Ub6) (linear) BML-UW0795 25µg 51.3kDa
Hepta-ubiquitin (Ub7) (linear) BML-UW0800 25µg 59.8kDa
Octa-ubiquitin (Ub8) (linear) BML-UW0805 25µg 68.4kDa
Nona-ubiquitin (Ub9) (linear) BML-UW0810 25µg 76.9kDa
Deca-ubiquitin (Ub10) (linear) BML-UW0815 25µg 86.5kDa
Undeca-ubiquitin (Ub11) (linear) BML-UW0820 25µg 94.0kDa

 
Formulation:Liquid. In 50mM TRIS, pH 8.0, containing 50mM sodium chloride.
 
Concentration:1mg/ml, except Ub4 and Ub6 which are supplied at 0.5mg/ml
 
Use/Stability:Stable for at least 12 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Source:Produced in E. coli.
 
Scientific Background:The post-translational modification of proteins with polyubiquitin chains occurs in a wide range of signalling pathways and is tightly regulated in order to ensure cellular homeostasis. The function, processing and ultimate fate of polyubiquitinylated proteins is thought to be determined by the nature of the linkage between adjacent ubiquitin molecules in the polyubiquitin chain.

In addition to lysine-linked polyubiquitin chains the amino terminus of ubiquitin can be used to form head-to-tail polyubiquitin chains, in which the C-terminal Gly of one ubiquitin is conjugated to the N-terminal Met of an adjacent ubiquitin. Such linear polyubiquitin chains are structurally similar to Lys63-linked polyubiquitin, despite the chemical differences between the two linkage types.

Several ubiquitin polygenes are encoded in eukaryotic cells and undergo post-translational processing to generate the cellular source of free ubiquitin monomers. However, linear polyubiquitin chains,in which the C-terminal Gly of one ubiquitin is conjugated to the N-terminal Met of an adjacent ubiquitin, have been shown to be assembled in vitro by an E3 ligase complex, known as the linear ubiquitin chain assembly complex (LUBAC), and ubiquitin binding domains (UBDs) with a preference for linear polyubiquitin have been identified in a number of proteins. A possible role for linear polyubiquitin modification in NF-κB pathway activation, involving linear polyubiquitinylation of NEMO, has also been reported.

Such observations suggest that linear polyubiquitin modification of proteins may play an important role in cellular processes in addition to that of lysine-linked polyubiquitin protein conjugation.
 

Product Literature References

Poly-ubiquitin drives the molecular interactions of NF-κB essential modulator by allosteric regulation: D.A. Catici, et al.; J. Biol. Chem. 290, 14130 (2015), Application(s): Fluorescent reporter signalling, Abstract; Full Text

General Literature References

Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation. : F. Tokunaga et al. ; Nat.Cell Biol. 11, 123 (2009), Abstract;
Molecular discrimination of structurally equivalent Lys 63-linked and linear polyubiquitin chains. : D. Komander et al. ; EMBO Rep. (2009), Abstract;
Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation.: S. Rahighi et al.; Cell 136, 1098 (2009), Abstract;
Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. : L. Jin et al.; Cell 133, 653 (2008), Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling.: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
A ubiquitin ligase complex assembles linear polyubiquitin chains. : T. Kirisako et al.; EMBO J. 25, 4877 (2006), Abstract; Full Text
Intracellular protein degradation: from a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting. : A. Ciechanover; Exp.Biol.Med. 231, 1197 (2006), Abstract; Full Text
Distinct monoubiquitin signals in receptor endocytosis.: K. Haglund et al. ; Trends Biochem.Sci. 28, 598 (2003), Abstract;
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.: M. H. Glickman & A. Ciechanover ; Physiol. Rev. 82, 373 (2002), Abstract; Full Text

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Tri-ubiquitin (linear) 

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Tetra-ubiquitin (linear) 

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Penta-ubiquitin (linear) 

Produced in E. coli., ≥95% (SDS-PAGE) | Print as PDF
 
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Hexa-ubiquitin (linear) 

Produced in E. coli., ≥95% (SDS-PAGE) | Print as PDF
 
BML-UW0795-0100 100 µg 129.00 USD
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Hepta-ubiquitin (linear) 

Produced in E. coli., ≥95% (SDS-PAGE) | Print as PDF
 
BML-UW0800-0100 100 µg 129.00 USD
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Octa-ubiquitin (linear) 

Produced in E. coli., ≥90% (SDS-PAGE) | Print as PDF
 
BML-UW0805-0100 100 µg 129.00 USD
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Nona-ubiquitin (linear) 

Produced in E. coli., ≥90% (SDS-PAGE) | Print as PDF
 
BML-UW0810-0100 100 µg 129.00 USD
Do you need bulk/larger quantities?
 

Deca-ubiquitin (linear) 

Produced in E. coli., ≥90% (SDS-PAGE) | Print as PDF
 
BML-UW0815-0100 100 µg 129.00 USD
Do you need bulk/larger quantities?
 

Undeca-ubiquitin (linear) 

Produced in E. coli., ≥90% (SDS-PAGE) | Print as PDF
 
BML-UW0820-0100 100 µg 128.00 USD
Do you need bulk/larger quantities?
 

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