Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

([K48-only]Ub)n-ubiquitinylated substrate

 
BML-UW0640-0025 25 µg 232.00 USD
Do you need bulk/larger quantities?
 

Product Specification

MW:~25-150kDa
 
Source:Protein components produced in E. coli. Synthesized enzymatically in vitro. (K48-only)Ubn-ubiquitinylated substrate protein was produced using K48-only mutant ubiquitin.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 150mM NaCl and 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
 
Quality Control:SDS-PAGE: Multiple high molecular weight bands are observed for (K48-only) Ub conjugate reactions (Fig. 1).
Immunoblotting: Detection of high molecular weight bands with the polyubiquitin-chain reactive monoclonal antibody FK2 (Prod. No. BML-PW8810, 1:1000 dilution).
 
Application Notes:Uses:
1. Deubiquitinylating enzyme substrates (general/linkage specific).
2. Profiling of DUB linkage-type preference or specificity in combination with other single lysine only polyubiquitinylated substrates (e.g. (K11-only)Ubn-ubiquitinylated substrate, Prod. No. BML-UW0620)
3. Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:Polyubiquitin chains, linked through specific lysine residues, are useful tools for investigating the specificity and reactivity of deubiquitinylating enzymes (DUBs) and the recognition and interaction of polyubiquitin modified proteins with ubiquitin binding domain (UBDs) containing proteins.
 
BML-UW0640 SDS-PAGE
Figure 1: Coomassie stained SDS-PAGE gel showing ubiquitinylation of substrate protein (X) with (K48-only) Ub to give (K48-only)Ubn-ubiquitinylated conjugates (Z; Prod. No. BML-UW0640).

BML-UW0640 USP2 assay WB
Figure 3: Western Blot showing (K48-only)Ubn-ubiquitinylated substrate (Prod. No. BML-UW0640) incubated with (+) or without (-) USP2cd (Prod. No. BML-UW9850), detected with PAb to ubiquitin-protein conjugates.
Please mouse over
BML-UW0640 SDS-PAGE BML-UW0640 USP2 assay WB

General Literature References

Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex: L. Jin, et al.; Cell 133, 653 (2008), Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
Intracellular protein degradation: from a vague idea thru the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting: A. Ciechanover; Exp. Biol. Med. (Maywood) 231, 1197 (2006), Abstract;
Distinct monoubiquitin signals in receptor endocytosis: K. Haglund, et al.; Trends Biochem. Sci. 28, 598 (2003), Abstract;
The BRCA1/BARD1 heterodimer assembles polyubiquitin chains through an unconventional linkage involving lysine residue K6 of ubiquitin: F. Wu-Baer, et al.; J. Biol. Chem. 278, 34743 (2003), Abstract;
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction: M.H. Glickman & A. Ciechanover; Physiol. Rev. 82, 373 (2002), Abstract;

Related Products

Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) 

Gold standard antibody for detection of ubiquitinylated proteins.
ELISA, IHC, IP, WB | Print as PDF
 
BML-PW8810-0100 100 µg 96.00 USD
 
BML-PW8810-0500 500 µg 323.00 USD
Do you need bulk/larger quantities?
 

USP2 (catalytic domain) (rat), (recombinant) (untagged) 

Produced in E. coli BL21 (λDE3). Untagged USP2 catalytic domain., ≥95% (SDS-PAGE, following ion exchange chromatography) | Print as PDF
 
BML-UW9850-0100 100 µg 258.00 USD
Do you need bulk/larger quantities?
 

([K11-only]Ub)n-ubiquitinylated substrate 

Protein components produced in E. coli. Synthesized enzymatically in vitro. (K11-only)Ubn-ubiquitinylated substrate protein was produced using K11-only mutant ubiquitin., ≥95% (SDS-PAGE) | Print as PDF
 
BML-UW0620-0025 25 µg 232.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,