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UbcH5b (human), (recombinant) (untagged)

BML-UW0565-0100 100 µg 267.00 USD
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Product Specification

Alternative Name:Ubc4, E2(17)KB2, Ubiquitin-conjugating enzyme E2 D2
Source:Produced in E. coli BL21 (λDE3).
UniProt ID:P62837
Formulation:Liquid. In 50mM HEPES, pH 6.5, containing 50mM NaCl and 1mM DTT.
Purity:≥95% (SDS-PAGE)
Purity Detail:Purified by ion exchange chromatography.
Application Notes:Suggested uses:
  1. Ubiquitinylation of specific proteins in vitro.
  2. Demonstrate novel proteins are potential targets for ubiquitinylation in vitro.
  3. Generate substrates for deubiquitinylating enzymes.
  4. Ubiquitinylation pathway studies.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Use/Stability:Stable for at least 12 months after receipt when stored at -80°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Scientific Background:UbcH5B, a homolog of the critically important yeast UBC4/5 family of proteins, is a ubiquitin-conjugating enzyme (E2) involved in the ubiquitin modification and subsequent proteasomal degradation of many cellular proteins in eukaryotes. UbcH5B is known to be essential for degradation of many regulatory and abnormal proteins. Together with UbcH5A and UbcH5C, UbcH5B forms one of the most active classes of E2 enzymes. They are associated with the degradation of a number of important human transcription factors, such as p53 and NF-κB, as well as a variety of non-proteolytic ubiquitin mediated processes such as signal transduction and endocytosis.
Left: SDS-PAGE image of untagged UbcH5b (Coomassie). Lane 1: MW markers (top to bottom) 116, 97, 84, 66, 55, 45, 36, 29, 24, 20, 14.2. Lane 2: BML-UW0565 (5µg). Right: Western blot of UbcH5b thioester activity assay in the presence (+) and absence (-) of ATP cofactor. Biotinylated-ubiquitin-enzyme conjugates were detected by Western blotting using Streptavidin-HRP.
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General Literature References

Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science 315, 201 (2007), Abstract;
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction: M.H. Glickman & A. Ciechanover; Physiol. Rev. 82, 373 (2002), Abstract;
Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha: H. Gonen, et al.; J. Biol. Chem. 274, 14823 (1999), Abstract;
Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53: M. Scheffner, et al.; PNAS 91, 8797 (1994), Abstract;
Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins: W. Seufert & S. Jentsch; EMBO J. 9, 543 (1990), Abstract;

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