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SUMO-1 (93-97)-AMCA

 
BML-UW0500-1000 1 mg 151.00 USD
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SUMO1-AMCA is a synthetic AMCA-peptide substrate based on the C-terminal residues of SUMO1 and is a useful fluorogenic substrate for deSUMOylating enzymes, including SENP1 (BML-UW9760). It has particular utility for the study of deSUMOylating activity where continuous monitoring of activity is essential. It provides an alternative substrate to SUMO1-AMC.

Product Specification

Sequence:E93QTGG97-AMCA
 
MW:747
 
Formulation:Lyophilized solid.
 
Purity:≥95% (HPLC)
 
Application Notes:Useful fluorogenic substrate for deSUMOylating enzymes.

Typical assay conditions:
Assay substrate concentration: 12.5µM-100µM.
Enzyme concentrations: 0.5µM-4µM.
Release of AMCA fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths).

Uses:
  1. Substrate for deSUMOylating enzyme activity assays.
  2. Identification/confirmation of enzyme deSUMOylation activity.
  3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC/AMCA substrates (e.g. SUMO1-AMC, NEDD8-AMCA).
 
Solubility:Soluble in DMSO. Can be subsequently diluted in aqueous buffers.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Store solid at -20°C for up to twelve months. Solutions can be stored for up to three months at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
bml-uw0500 assay
DeSUMOylation Assay: 2µM SENP1 (Prod. No. BML-UW9760); 100µM SUMO1-AMCA (Prod. No. BML-UW0500); 50mM TRIS, pH 8.0, 20mM NaCl, 5mM DTT.
bml-uw0500 hplc
HPLC trace
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bml-uw0500 assay bml-uw0500 hplc

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