USP25 (isoform 2) (human), (recombinant) (His-tag)



BML-UW0475-0050	50 µg	108.00 USD

Product Specification

Alternative Name:	Ubiquitin thioesterase 25, Ubiquitin-specific protease 25, Deubiquitinating enzyme 25, USP on chromosome 21

Concentration:	0.116mg/ml

MW:	~124kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In 50mM TRIS, pH 8.0, containing 50mM NaCl and 1mM DTT.

EC:	3.1.2.15

Source/Host:	Produced in E. coli.

Application:	1. Deubiquitinylation assays using small molecule substrates (e.g. Ub-AMC, Prod. No. BML-SE211) or ubiquitin-protein conjugates. 2. Studies of the effect of SUMO modification on USP25 activity. 3. Investigation of the role of USP25 in various biological processes. 4. Development of inhibitors for USP25 deubiquitinylating activity.

Short Term Storage:	-80°C

Long Term Storage:	-80°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -80°C.

Handling:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Ubiquitin specific protease-25 (USP25) is a vertebrate specific deubiquitinating enzyme. Three alternative splice variants have been identified; two ubiquitously expressed and one, the longer isoform USP25m, restricted to expression in muscular tissues where it is upregulated during myogenesis. Whilst its biological function and regulation have yet to be fully elucidated, increased USP25 expression levels have been observed in Down syndrome fetal brains and in breast cancer tissue. USP25 is also a target for SUMO modification, which takes place within its two ubiquitin intreaction motifs and has been shown to impair it’s binding to and hydrolysis of polyubiquitin chains.

Background / Technical Information:	UniProt ID Q9UHP3: Ubiquitin carboxyl-terminal hydrolase 25

Figure 1: SDS-PAGE image of His₆-tagged USP25 isoform 2 (Coomassie). Lane 1: Prod. No. BML-UW0475 (1µg). Lane 2: MW markers.

Figure 2: DUB Assay: 50nM His₆-tagged USP25 isoform 2 (Prod. No. BML-UW0475); 500nM Ubiquitin-AMC; 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT.

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General Literature References

Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25: E. Meulmeester, et al.; Mol. Cell 30, 610 (2008), Abstract;

Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics: S. Deng, et al.; Breast Cancer Res. Treat. 104, 21 (2007), Abstract;

The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins: A. Bosch-Comas, et al.; Cell Mol. Life Sci. 63, 723 (2006), Abstract;

Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25: R. Valero, et al.; Genome Biol. 2, RESEARCH0043 (2001), Abstract;

USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2: R. Valero, et al.; Genomics 62, 395 (1999), Abstract;