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USP25 (isoform 2) (human), (recombinant) (His-tag)

 
BML-UW0475-0050 50 µg 129.00 USD
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Product Specification

Alternative Name:Ubiquitin thioesterase 25, Ubiquitin-specific protease 25, Deubiquitinating enzyme 25, USP on chromosome 21
 
MW:~124kDa
 
Source:Produced in E. coli.
 
EC:3.1.2.15
 
UniProt ID:Q9UHP3
 
Formulation:Liquid. In 50mM TRIS, pH 8.0, containing 50mM NaCl and 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Application Notes:Uses:
1. Deubiquitinylation assays using small molecule substrates (e.g. Ub-AMC, Prod. No. BML-SE211) or ubiquitin-protein conjugates.
2. Studies of the effect of SUMO modification on USP25 activity.
3. Investigation of the role of USP25 in various biological processes.
4. Development of inhibitors for USP25 deubiquitinylating activity.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:Ubiquitin specific protease-25 (USP25) is a vertebrate specific deubiquitinating enzyme. Three alternative splice variants have been identified; two ubiquitously expressed and one, the longer isoform USP25m, restricted to expression in muscular tissues where it is upregulated during myogenesis. Whilst its biological function and regulation have yet to be fully elucidated, increased USP25 expression levels have been observed in Down syndrome fetal brains and in breast cancer tissue. USP25 is also a target for SUMO modification, which takes place within its two ubiquitin intreaction motifs and has been shown to impair it’s binding to and hydrolysis of polyubiquitin chains.
 
bml-uw0475 usp25 sdspage-2
Figure 1: SDS-PAGE image of His6-tagged USP25 isoform 2 (Coomassie). Lane 1: Prod. No. BML-UW0475 (1µg). Lane 2: MW markers.
bml-uw0475
Figure 2: DUB Assay: 50nM His6-tagged USP25 isoform 2 (Prod. No. BML-UW0475); 500nM Ubiquitin-AMC; 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT.
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bml-uw0475 usp25 sdspage-2 bml-uw0475

Product Literature References

USP7 is essential for maintaining Rad18 stability and DNA damage tolerance: A. Zlatanou, et al.; Oncogene 35, 965 (2016), Application(s): Cell Culture, Abstract;

General Literature References

Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25: E. Meulmeester, et al.; Mol. Cell 30, 610 (2008), Abstract;
Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics: S. Deng, et al.; Breast Cancer Res. Treat. 104, 21 (2007), Abstract;
The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins: A. Bosch-Comas, et al.; Cell Mol. Life Sci. 63, 723 (2006), Abstract;
Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25: R. Valero, et al.; Genome Biol. 2, RESEARCH0043 (2001), Abstract;
USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2: R. Valero, et al.; Genomics 62, 395 (1999), Abstract;

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Ubiquitin-AMC 

High purity industry standard substrate for deubiquitinylating enzymes (DUBs).
Produced in E.coli., ≥95% (HPLC) | Print as PDF
 
BML-SE211-0025 25 µg 192.00 USD
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