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Rbx1 (human), (recombinant) (His-tag)

 
BML-UW0395-0025 25 µg 313.00 USD
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Product Specification

Alternative Name:Ring box protein-1, ROC1, Regulator of cullins 1, Ring finger protein 75
 
MW:~14kDa
 
Source:Produced in E. coli BL21 (λDE3).
 
UniProt ID:P62877
 
Formulation:Liquid. In 50mM TRIS, pH 8.0, containing 50mM NaCl and 1mM DTT.
 
Purity:≥98% (SDS-PAGE)
 
Purity Detail:Purified by nickel sepharose affinity chromatography.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 12 months after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:Cullin-based E3 ligases, such as SCF (Skp1-Cdc53-F-box protein), are a large family of multi-subunit ubiquitin ligases with diverse cellular functions, including the regulation of the cell cycle, of the DNA damage response, and of various transcription factors. These ligases are composed of one of six mammalian cullin homologs (Cul1, Cul2, Cul3, Cul4a, Cul4b, and Cul5), the Ring finger containing protein Rbx1/Roc1, and cullin homolog-specific adaptor and substrate recognition subunits. Activation of cullin-based ligases require the covalent modification of a conserved lysine residue in the cullin protein with the ubiquitin-like protein NEDD8. This modification is catalyzed by the Rbx1 subunit.
 
bml-uw0395 coomassie
Figure: SDS-PAGE image of Rbx1, N-terminally His6 tagged (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2, 6.5 kDa. Lane 2: Rbx1 (human) (recombinant) (His) (Prod. No. BML-UW0395) (2 µg).
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bml-uw0395 coomassie

General Literature References

Substrate-mediated regulation of cullin neddylation: E.H. Chew & T. Hagen; J. Biol. Chem. 282, 17032 (2007), Abstract;
A hitchhiker’s guide to the cullin ubiquitin ligases: A.R. Willems, et al.; Biochim. Biophys. Acta 1695, 133 (2004), Abstract;
Nedd8 on cullin: building an expressway to protein destruction: Z.Q. Pan, et al.; Oncogene 23, 1985 (2004), Abstract;

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