Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

[K11R]SUMO-3 (human), (recombinant) (GST-tag)

 
BML-UW0385-0100 100 µg 89.00 USD
Do you need bulk/larger quantities?
 
The [K11R] mutant of SUMO-3 is incapable of forming polySUMO-3 chains at Lys11 and can be used to selectively mono-/multi-SUMOylate target proteins.

Product Specification

Alternative Name:SMT3A, SMT3H1
 
MW:~40kDa
 
Source:Produced in E. coli BL21 (λDE3).
 
UniProt ID:P55854
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 150mM NaCl and 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by glutathione affinity chromatography.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:SUMO (small ubiquitin-related modifier) is a member of a family of ubiquitin-like proteins involved in the regulation of the cellular functions of a wide variety of proteins. Four members of the SUMO family have been described in vertebrates, SUMO-1 and the close homologues SUMO-2 and SUMO-3, with some 50% homology between SUMO-1 and SUMO-2/3.
 
bml-uw0385 coomassie
Figure: SDS-PAGE image of [K11R]SUMO-3, GST-tagged (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2KDa. Lane 2: Prod. No. BML-UW0385 (4µg).
Please mouse over
bml-uw0385 coomassie

General Literature References

A stress-dependent SUMO4 sumoylation of its substrate proteins: W. Wei, et al.; BBRC 375, 454 (2008), Abstract;
The SUMO isopeptidase Ulp2 prevents accumulation of SUMO chains in yeast: G.R. Bylebyl, et al.; J. Biol. Chem. 278, 44113 (2003), Abstract;
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem. 276, 35368 (2001), Abstract;
SUMO, ubiquitin’s mysterious cousin: S. Muller, et al.; Nat. Rev. Mol. Cell Biol. 2, 202 (2001), Abstract;
SUMO-1 conjugation in vivo requires both a consensus modification motif and nuclear targeting: M.S. Rodriguez, et al.; J. Biol. Chem. 276, 12654 (2001), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
SUMO
By biological activity:
SUMO Modified protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,