IRF2 (human), (recombinant) (His-tag)



BML-UW0335-0100	100 µg	209.00 USD

Product Specification

Alternative Name:	Interferon regulatory factor 2

Concentration:	0.5mg/ml

Purity Detail:	Purified by Ni-affinity chromatography.

MW:	~43kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In 50mM TRIS, pH 7.5, containing 50mM NaCl and 1mM DTT.

Source/Host:	Produced in E. coli BL21 (λDE3).

Application:	1. Substrate for in vitro SUMOylation reactions. 2. Investigation of SUMO conjugation pathway and SUMO chain formation.

Short Term Storage:	-80°C

Long Term Storage:	-80°C

Use/Stability:	Stable for at least 12 months after receipt when stored at -80°C.

Handling:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	IRF2 interacts with the SUMO-E3 ligase PIASy and is SUMOylated in vivo. This SUMO modification regulates the transcriptional activities of IRF2 with mutagenetic analysis suggesting that it contains three SUMOylation sites. SUMOylation of IRF2 has no significant effects on its nuclear localization or DNA binding activity, but increases its ability to inhibit IRF1 transcriptional activity and decreases its ability to activate the ISRE and histone H4 promoters.

Background / Technical Information:	UniProt ID P14316: Interferon regulatory factor 2 (human)

Figure 1: SDS-PAGE image of His₆-IRF2 (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20 kDa. Lane 2: IRF2 (human) (recombinant) (His) (Prod. No. BML-UW0335) (2µg).