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IRF2 (human), (recombinant) (His-tag)

 
BML-UW0335-0100 100 µg 252.00 USD
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Product Specification

Alternative Name:Interferon regulatory factor 2
 
MW:~43kDa
 
Source:Produced in E. coli BL21 (λDE3).
 
UniProt ID:P14316
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 50mM NaCl and 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by Ni-affinity chromatography.
 
Application Notes:Uses:
1. Substrate for in vitro SUMOylation reactions.
2. Investigation of SUMO conjugation pathway and SUMO chain formation.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 12 months after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:IRF2 interacts with the SUMO-E3 ligase PIASy and is SUMOylated in vivo. This SUMO modification regulates the transcriptional activities of IRF2 with mutagenetic analysis suggesting that it contains three SUMOylation sites. SUMOylation of IRF2 has no significant effects on its nuclear localization or DNA binding activity, but increases its ability to inhibit IRF1 transcriptional activity and decreases its ability to activate the ISRE and histone H4 promoters.
 
bml-uw0335 sdspage
Figure 1: SDS-PAGE image of His6-IRF2 (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20 kDa. Lane 2: IRF2 (human) (recombinant) (His) (Prod. No. BML-UW0335) (2µg).
bml-uw0335 wb
Figure 2: Western blot of IRF2 SUMOylation assay. SUMOylated proteins detected using SUMO-2/3 antibody (Prod. No. BML-PW9465, 1:1'000).
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bml-uw0335 sdspage bml-uw0335 wb

General Literature References

Regulation of IRF2 transcriptional activity by its sumoylation: K.J. Han, et al.; BBRC 372, 772 (2008), Abstract;
TLR signalling and activation of IRFs: revisiting old friends from the NF-kappaB pathway: P.N. Moynagh; Trends Immunol. 26, 469 (2005), Abstract;
IRF family of transcription factors as regulators of host defense: T. Taniguchi, et al.; Annu. Rev. Immunol. 19, 623 (2001), Abstract;

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