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Ubiquitin (human), (recombinant)

 
BML-UW0280-0001 1 mg 41.00 USD
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Product Specification

MW:~8.6kDa
 
Source:Produced in E. coli.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
 
Formulation:Lyophilized.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
 
Application Notes:For use in ubiquitinylation studies.
 
Reconstitution:Reconstitute in minimum volume of DMSO (5-10µL) followed by dilution to required concentration with aqueous buffer of choice (e.g. 1mg/mL in PBS).
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 12 months after receipt when stored at -20°C.
 
Scientific Background:The covalent attachment of ubiquitin to proteins (ubiquitinylation) and their subsequent proteasomal degradation plays a fundamental role in the regulation of cellular function through biological events involving cell cycle, differentiation, immune responses, DNA repair, chromatin structure, and apoptosis.

Ubiquitinylation is achieved through three enzymatic steps. In an ATP-dependent process, the ubiquitin activating enzyme (E1) catalyzes the formation of a reactive thioester bond with ubiquitin, followed by its subsequent transfer to the active site cysteine of a ubiquitin carrier protein (E2). The specificity of ubiquitin ligation arises from the subsequent association of the E2-ubiquitin thioester with a substrate specific ubiquitin-protein isopeptide ligase (E3), which facilitates the formation of the isopeptide linkage between ubiquitin and its target protein.
 

General Literature References

Mechanisms underlying ubiquitination: C.M. Pickart, et al.; Annu. Rev. Biochem. 70, 503 (2001), Abstract;
The ubiquitin-proteasome system and endocytosis: G.J. Strous & R. Govers; J. Cell Sci. 112 (Pt 10), 1417 (1999), Abstract;
The ubiquitin system: A. Hershko, et al.; Annu. Rev. Biochem. 67, 425 (1998), Abstract;
Pathways of ubiquitin conjugation: A. L. Haas et al.; Faseb. J. 11, 1257 (1997), Abstract;

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