Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Hdm2 (catalytic RING domain) (human), (recombinant) (GST-tag)

 
BML-UW0200-0025 25 µg 168.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Human double minute 2 protein
 
MW:~35kDa
 
Source:Produced in E. coli BL21 (λDE3).
 
UniProt ID:Q00987
 
Formulation:Liquid. In TBS, pH 7.5, containing 150mM sodium chloride and 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by glutathione affinity chromatography.
 
Activity:Modifies p53 (Prod. No. BML-FW8820) in ubiquitinylation reactions containing Ube1 (Prod. No. BML-UW9410), UbcH5b (Prod. No. BML-UW9060) and energy regeneration solution.
 
Application Notes:Uses:
1.in vitro generation of ubiquitinylated p53 for use in Ub-p53 related studies.
2.Investigation of the p53 ubiquitin modification process itself.
3.Ubiquitin modification of other known Hdm2 substrate proteins.
4.NEDD8 modification of p53.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:p53 is a much studied and complex multifunctional protein, which plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis, through its involvement in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for these processes. Activation and regulation of the p53 transcription pathway is controlled by a range of post-translational modifications including acetylation, phosphorylation and ubiquitinylation.  In normal cells, p53 is maintained at a low level mainly through Hdm2-mediated ubiquitinylation and subsequent degradation by the proteasome. Hdm2 is a RING domain dependent ubiquitin E3 ligase that utilizes its C-terminal RING domain to promote not only p53 ubiquitinylation, predominantly at the C-terminus of p53, but also to target Hdm2 itself for auto-ubiquitinylation and subsequent degradation. The isolated Hdm2 C-terminal RING domain (residues 418-491) has been shown to be sufficient for both p53 and self-ubiquitinylation activity.
 
BML-UW0200 SDS-PAGE
SDS-PAGE analysis: Lane 1: 2μg GST-Hdm2 catalytic RING domain (Prod. No. BML-UW0200). Lane 2: MW markers (top to bottom) 116, 97, 84, 66, 55, 45, 36, 29, 24, 20.
BML-UW0200 WB
Activity analysis: Hdm2 catalytic RING domain ubiquitinylation of p53 (Prod. No. BML-FW8820) +/- Hdm2 RING domain. p53 species detected by western blotting using p53 monoclonal antibody (Prod. No. BML-PW1085).
Please mouse over
BML-UW0200 SDS-PAGE BML-UW0200 WB

General Literature References

Solution Structure of the Hdm2 C2H2C4 RING, a Domain Critical for Ubiquitination of p53: M. Kostic, et al.; J. Mol. Biol. (2006),
Ubiquitination, phosphorylation and acetylation: the molecular basis for p53 regulation: C.L. Brooks, et al.; Curr. Opin. Cell Biol. 15, 164 (2003),
Deconstruction of p53 functions and regulation: Y. Haupt, et al.; Oncogene 21, 8223 (2002),
Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53: S. Fang, et al.; J. Biol. Chem. 275, 8945 (2000),
Multiple lysine mutations in the C-terminal domain of p53 interfere with MDM2-dependent protein degradation and ubiquitination: S. Nakamura, et al.; Mol. Cell Biol. 20, 9391 (2000),
Regulation of the p53 tumor suppressor protein: M. Oren, et al.; J. Biol. Chem. 274, 36031 (1999),
Mdm2 promotes the rapid degradation of p53: Y. Haupt, et al.; Nature 387, 296 (1997),
Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene: G. Matlashewski, et al.; EMBO J. 3, 3257 (1984),

Related Products

p53 (human), (recombinant) (His-tag) 

Produced in insect cells using a baculovirus system. Full length human p53 is fused to a His-tag. | Print as PDF
 
BML-FW8820-0020 20 µg 400.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

Brochures
Essential Research Tools for Neurodegeneration & Neural Signaling
Essential Research Tools for Neurodegeneration & Neural Signaling
Download as PDF

Technical Posters
Disease-Associated Stress Signaling
Disease-Associated Stress Signaling
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,