C-Terminal mutant of mature SUMO-1 protein for use in proteomic investigations. The use of this mutant SUMO-1 for the modification of target proteins, with recovery of resulting SUMO conjugates using the GST-tag, followed by tryptic digestion gives rise to peptides with a SUMO specific side chain signature not readily obtainable using wild-type SUMO modification. This modification allows ready differentiation by mass spectrometric analysis of SUMO modifications from those derived from ubiquitin and other Ubls.
Product Specification
| Source: | Produced in E. coli. |
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| UniProt ID: | P63165 |
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| Formulation: | Liquid. In 50mM TRIS, pH 7.6, containing 1mM EDTA. |
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| Application Notes: | Uses:
Identification of SUMO modified proteins by proteomic methods.
Identification of SUMO modification sites by proteomic methods. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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