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Somatostatin polyclonal antibody

 
BML-SZ1116-0025 25 µl 345.00 USD
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Product Specification

Host:Rat
 
Immunogen:Somatostatin-28.
 
UniProt ID:P61278
 
Species reactivity:Species independent
 
Specificity:Recognizes somatostatin in a wide range of species.
 
Applications:IHC (FS), IHC (PS)
 
Formulation:Liquid serum which has been clarified, but not purified, and contains 0.01M sodium azide.
 
Use/Stability:Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
 
Handling:Avoid freeze/thaw cycles. Aliquot undiluted antibody into smaller volumes (not less than 10µl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Since the discovery of somatostatin-14 (SOM-14) in ovine hypothalamus, several other forms of somatostatin-like immunoreactants of different molecular weight have been reported, including somatostatin-28 which expresses the full SOM-14 sequence at its C-terminus (SOM-28[15-28]). In mammals both molecular forms are encoded at the C-terminus of the somatostatin precursor molecule. Hundreds of immunolocalisation studies have been published demonstrating the existence of somatostatin-like molecules in neurons, in pancreatic islet D cells and in endocrine cells distributed throughout the mammalian gut, as well as in rare endocrine tumours (so-called somatostatinomas). The molecular forms appear to be co-packaged in dense-cored secretory granules, frequently with other transmitter substances. A recent paper has shown that the majority of somatostatin-containing axons in the rat spinal cord express vesicular gluatamate transporter protein-2 (VGLUT2).
 
Protocol:Test tissues: Tissues from many animal species can be used. It is not recommended to use this antiserum to detect somatostatin in rat tissues, and care should be taken to ensure that when used on mouse tissue the secondary antiserum will not cross-react with rat immunoglobulin.

Fixatives: Fixation by transcardial perfusion is best. For optimal tissue preservation and maintenance of antigenicity, use 4% buffered paraformaldehyde (4g paraformaldehyde heated to 70°C in 100ml 0.1M sodium phosphate buffer pH 7.2, allowed to cool). A short pre-wash (50-100ml) to remove blood is advised. Following dissection small pieces of tissue should be post-fixed for 30-60min in the same fixative at room temperature. Glutaraldehyde (up to 0.2%) can be added if required. Tissues unsuitable for perfusion should be fixed by immersion in the above fixative, but care should be taken to avoid over-fixation (small pieces of tissue do not require more than 60min in general).

Processing: Tissue treatment should be carried out as suggested:
  • Vibratome slices: Tissues should be rinsed in buffer (1h to 24h) before sectioning.
  • Cryostat sections: After washing in buffer, place tissue in 10-30% buffered sucrose overnight or until the tissue sinks.
  • Paraffin wax sections: A brief (10min) pre-treatment in trypsin solution (1mg/ml in PBS) dramatically enhances immunostaining.
Dilution range: 1:1000 or greater, using overnight incubation (at 4°C) and PAP/ABC-peroxidase procedure. 1:200 when using an overnight incubation at 4°C with an indirect immunofluorescence method.
 

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