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Somatostatin polyclonal antibody

 
BML-SZ1114-0100 100 µl 310.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Somatostatin-28.
 
UniProt ID:P61278
 
Species reactivity:Species independent
 
Specificity:Recognizes somatostatin in a wide range of species.
 
Applications:IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1:1,000)
Immunohistochemistry (paraffin sections, 1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.05% sodium azide.
 
Use/Stability:Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
 
Handling:Avoid freeze/thaw cycles. Aliquot undiluted antibody into smaller volumes (not less than 10µl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Since the discovery of somatostatin-14 (SOM-14) in ovine hypothalamus, several other forms of somatostatin-like immunoreactants of different molecular weight have been reported, including somatostatin-28 which expresses the full SOM-14 sequence at its C-terminus (SOM-28[15-28]). In mammals both molecular forms are encoded at the C-terminus of the somatostatin precursor molecule. Hundreds of immunolocalisation studies have been published demonstrating the existence of somatostatin-like molecules in neurons, in pancreatic islet D cells and in endocrine cells distributed throughout the mammalian gut, as well as in rare endocrine tumours (so-called somatostatinomas). The molecular forms appear to be co-packaged in dense-cored secretory granules, frequently with other transmitter substances. It has been shown that the majority of somatostatin-containing axons in the rat spinal cord express vesicular gluatamate transporter protein-2 (VGLUT2).
 
Protocol:Test tissues: Tissues from many animal species can be used.

Fixatives: Fixation by transcardial perfusion is best. For optimal tissue preservation and maintenance of antigenicity, use 4% buffered paraformaldehyde (4g paraformaldehyde heated to 70°C in 100ml 0.1M sodium phosphate buffer, pH 7.2, allowed to cool). A short pre-wash (50-100ml) to remove blood is advised. Following dissection small pieces of tissue should be post-fixed for 30-60min in the same fixative at room temperature. Glutaraldehyde (up to 0.2%) can be added if required. Tissues unsuitable for perfusion should be fixed by immersion in the above fixative, but care should be taken to avoid over-fixation (small pieces of tissue do not require more than 60min in general).

Processing: Tissue treatment should be carried out as suggested:
  • Vibratome slices: Tissues should be rinsed in buffer (1h to 24h) before sectioning.
  • Cryostat sections: After washing in buffer, place tissue in 10-30% buffered sucrose overnight or until the tissue sinks.
  • Paraffin wax sections: A brief (10min) pre-treatment in trypsin solution (1mg/mL in PBS) dramatically enhances immunostaining.
Dilution range: 1:1000 or greater, using overnight incubation (at 4°C) and PAP/ABC-peroxidase procedure. 1:200 when using an overnight incubation at 4°C with an indirect immunofluorescence method.
 

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