Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 


Inhibitor of PLC activation
BML-ST391-0005 5 mg 98.00 USD
BML-ST391-0025 25 mg 385.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ALX-370-005

Inhibits agonist induced phospholipase C (PLC) activation (IC50=1-2.1 µM) in human platelets. It also inhibits agonist induced down-regulation of muscarinic receptors in SK-N-SH neuroblastoma cells. It is a useful tool to investigate receptor-mediated PI turnover in signal transduction. It is a potent inhibitor of human neutrophil adhesion to biological surfaces as well as adhesion-dependent granule exocytosis and oxidative burst. Inhibits 5-lipoxygenase in vivo and in vitro (IC50 = 30nM for recombinant enzyme and 2.4µM for PMNL homogenates).

Product Specification

Alternative Name:1-(6-((17β-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
Purity:≥98% (HPLC)
Appearance:white to off-white solid
Solubility:Soluble in methylene chloride or chloroform. Slightly soluble in DMSO (2mg/ml) or 100% ethanol (1mg/ml), sparingly soluble in aqueous solutions. 
Long Term Storage:Ambient
Use/Stability:U73122 is only sparingly soluble in aqueous media. Therefore the compounds are delivered to cells either after dissolving in organic solvents or complexing with serum proteins. Serum albumin (1-10mg/ml).

Dissolve a weighed amount of U-73122 in methylene chloride or chloroform. Dispense in aliquots sufficient for one experiment into suitable vessels. Evaporate solvent with nitrogen.  

Stock solutions in DMSO or ethanol are stable for 2 months when stored at -20°C. Preferably dissolve just before use. Use caution in reusing stored DMSO solutions. Discard solutions that have turned to a pink color, which indicates a loss of inhibitor activity. Dried aliquots prepared from chloroform solutions are stable for up to 1 month when stored at -20°C.

Useful range of concentrations for U-73122 is 0.1 to 10μM.  The appropriate concentration will depend upon the cell type, cell density, medium concentration (the maleimide moiety of U-73122 may react with thiol containing compounds) and the particular cell parameter that is being measured.
BML-ST391 structure
Please mouse over
BML-ST391 structure

Product Literature References

1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) attenuates gemcitabine-induced neutrophil extravasation: J. Jeong, et al.; Cell Biosci. 9, 4 (2019), Abstract; Full Text
Effect of selective serotonin (5-HT)2B receptor agonist BW723C86 on epidermal growth factor/transforming growth factor-α receptor tyrosine kinase and ribosomal p70 S6 kinase activities in primary cultures of adult rat hepatocytes: K. Naito, et al.; Biol. Pharm. Bull. (2019), Application(s): used w. adult rat liver hepatocytes, Abstract; Full Text
VLDL-activated cell signaling pathways that stimulate adrenal cell aldosterone production: Y.Y. Tsai, et al.; Mol. Cell. Endocrinol. 433, 138 (2016), Application(s): Cell culture, Abstract;
Activation of phosphatidylinositol 3-kinase β by the platelet collagen receptors integrin α2β1 and GPVI: The role of Pyk2 and c-Cbl: D. Manganaro, et al.; Biochim. Biophys. Acta 1853, 1879 (2015), Application(s): Cell Culture, Abstract;
CXCL12 regulates platelet activation via the regulator of G-protein signaling 16: Z.A. Karim, et al.; Biochim. Biophys. Acta 1863, 314 (2015), Application(s): Cell culture, Abstract;
FGF9-induced changes in cellular redox status and HO-1 upregulation are FGFR-dependent and proceed through both ERK and AKT to induce CREB and Nrf2 activation: J.I. Chuang, et al.; Free Radic. Biol. Med. 89, 274 (2015), Application(s): Cell Culture, Abstract;
Serine-727 phosphorylation activates hypothalamic STAT-3 independently from tyrosine-705 phosphorylation: A. Breit, et al.; Mol. Endocrinol. 29, 445 (2015), Application(s): ELISA, Abstract; Full Text
Comparative Effects of the Endogenous Agonist Glucagon-Like Peptide-1 (GLP-1)-(7-36) Amide and the Small-Molecule Ago-Allosteric Agent "Compound 2" at the GLP-1 Receptor: K. Coopman, et al.; J. Pharmacol. Exp. Ther. 334, 795 (2010), Application(s): IP of human embryonic kidney cells, Abstract; Full Text
Regulation of constitutive cargo transport from the trans-Golgi network to plasma membrane by Golgi-localized G protein betagamma subunits: R. Irannejad & P.B. Wedegaertner; J. Biol. Chem. 285, 32393 (2010), Application(s): HeLa cells treatment, Abstract; Full Text
The aminosteroid phospholipase C antagonist U-73122 (1-[6-[[17-beta-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrro le-2,5-dione) potently inhibits human 5-lipoxygenase in vivo and in vitro: C. Feisst, et al.; Mol. Pharmacol. 67, 1751 (2005), Abstract;
U-73122: a potent inhibitor of human polymorphonuclear neutrophil adhesion on biological surfaces and adhesion-related effector functions: R.J. Smith, et al.; J. Pharmacol. Exp. Ther. 278, 320 (1996), Abstract;
A phospholipase C inhibitor, U-73122, blocks TSH-induced inositol trisphosphate production, Ca2+ increase and arachidonic acid release in FRTL-5 thyroid cells: X.D. Wang, et al.; Biochim. Biophys. Acta 1223, 101 (1994), Abstract;
U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells: A. Tatrai, et al.; Biochim. Biophys. Acta 1224, 575 (1994), Abstract;
Use of U-73122 as an Inhibitor of Phospholipase C-Dependent Processes: J.E. Bleasdale & S.K. Fisher; Neuroprotocols 3, 125 (1993), Full Text
U-73122, an aminosteroid phospholipase C antagonist, noncompetitively inhibits thyrotropin-releasing hormone effects in GH3 rat pituitary cells: R.C. Smallridge, et al.; Endocrinology 131, 1883 (1992), Abstract;
U73122 inhibits Ca2+ oscillations in response to cholecystokinin and carbachol but not to JMV-180 in rat pancreatic acinar cells: D.I. Yule, et al.; J. Biol. Chem. 267, 13830 (1992), Abstract;
The aminosteroid U-73122 inhibits muscarinic receptor sequestration and phosphoinositide hydrolysis in SK-N-SH neuroblastoma cells. A role for Gp in receptor compartmentation: A.K. Thompson, et al.; J. Biol. Chem. 266, 23856 (1991), Abstract;
Receptor-coupled signal transduction in human polymorphonuclear neutrophils: effects of a novel inhibitor of phospholipase C-dependent processes on cell responsiveness: R.J. Smith, et al.; J. Pharmacol. Exp. Ther. 253, 688 (1990), Abstract;
Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutrophils: J.E. Bleasdale, et al.; J. Pharmacol. Exp. Ther. 255, 756 (1990), Abstract;

Related Literature

Technical Posters
Arachidonic Acid Metabolites
Arachidonic Acid Metabolites
Download as PDF

All new literature pieces

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,