Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Cathepsin F (human), (recombinant)

Highly active
BML-SE568-0005 5 µg 390.00 USD
Do you need bulk/larger quantities?

Product Specification

Source:Produced in insect cells (secreted) as untagged proenzyme; activated during purification. Produced in a baculovirus expression system.
UniProt ID:Q9UBX1
Formulation:Liquid. In 20mM NaOAc, pH 5.0, containing 2.5mM EDTA, 250mM sodium chloride and 20mM L-cysteine.
Purity:≥80% (SDS-PAGE)
Purity Detail:Purified by multi-step chromatography.
Specific Activity:≥1 U/mg. One unit hydrolyzes 1 µmol Z-Phe-Arg-AMC substrate (OMNICATHEPSIN® Fluorogenic Substrate, Prod. No. BML-P139) per min. at 37°C.
Application Notes:Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Use/Stability:Stable for at least 6 months after receipt when stored as supplied at -80°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Scientific Background:Recombinant glycosylated procathepsin F cloned from human cDNA (NM_003793), expressed in insect cells, and purified as the active form of the enzyme. Cathepsin F, a member of the papain family of lysosomal cysteine proteases, acts upon proteins such as MHC Class II-associated invariant chain and ApoB-100. It is inhibited by the cysteine proteinase inhibitor E-64 (Prod. No. BML-EI105) and cystatin C (Prod. No. BML-SE479). It is a lysosomal protease expressed in numerous tissues, notably brain and macrophages. It functions in the immune system and is implicated in disease states such as atherosclerosis, cancer, and angiogenesis.

Product Literature References

Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease: J. Peters, et al.; Biochem. Biophys. Res. Commun. 457, 334 (2015), Application(s): Cell Culture, Western Blotting, Abstract;
Development of cell-active non-peptidyl inhibitors of cysteine cathepsins: D. Dana, et al.; Bioorg. Med. Chem. 21, 2975 (2013), Abstract;

General Literature References

Overexpression of cathepsin F, matrix metalloproteinases 11 and 12 in cervical cancer: G. Vazquez-Ortiz, et al.; BMC Cancer 5, 164 (2005), Abstract; Full Text
Cysteine protease cathepsin F is expressed in human atherosclerotic lesions, is secreted by cultured macrophages and modifies low density lipoprotein particles in vitro: K. Oorni; J. Biol. Chem. 279, 34776 (2004), Abstract; Full Text
Human cathepsin F: expression in baculovirus system, characterization and inhibition by protein inhibitors: M. Fonovic, et al. ; Biol. Chem. 385, 505 (2004), Abstract;
Expression, purification, crystallization and preliminary X-ray diffraction studies of human cathepsin F complexed with irreversible vinyl sulfone inhibitor: J.D. Ho, et al.; Acta Crystallogr. D. Biol. Crystallogr. D58, 2187 (2002), Abstract;
The crystal structure of human cathepsin F and its implications for the development of novel immunomodulators: J.R. Somoza, et al.; J. Mol. Biol. 322, 559 (2002), Abstract;
Human Cathepsins F and W: A new subgroup of cathepsins: T. Wex, et al.; Biochem. Biophys. Res. Commun. 259, 401 (1999), Abstract;
Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain: I. Santamaria, et al.; J. Biol. Chem. 274, 13800 (1999), Abstract; Full Text
Human cathepsin F: Molecular cloning , functional expression, tissue localization, and enzymatic characterization: B. Wang, et al.; J. Biol. Chem. 273, 32000 (1998), Abstract; Full Text

Related Products

SKF-525A . HCl 

Cytochrome P450 inhibitor
62-68-0, ≥95% (TLC) | Print as PDF
BML-EI105-0250 250 mg 90.00 USD
BML-EI105-1000 1 g 260.00 USD
Do you need bulk/larger quantities?

Cystatin C (human), (purified) 

Protease inhibitor
Isolated from human urine., ≥95% (SDS-PAGE) | Print as PDF
BML-SE479-0100 100 µg 205.00 USD
Do you need bulk/larger quantities?

OMNICATHEPSIN® fluorogenic substrate 

65147-22-0, ≥99% | Print as PDF
BML-P139-0010 10 mg 65.00 USD
BML-P139-0050 50 mg 232.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Cathepsin Enzyme
By catalog section:


Technical Service
Customer Service

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,