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Abl1 (mouse), (recombinant) (His-tag)

 
BML-SE563-0005 5 µg 187.00 USD
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Product Specification

Alternative Name:Abelson murine leukemia viral oncogene homolog 1
 
Sequence:Mouse recombinant Abl1 (aa 27-1123) with N-terminal histidine tag. Mouse Abl1 is 89% identical (91% similar) to human Abl1.
 
MW:~135kDa
 
Source:Produced in insect cells. Produced in a baculovirus expression system.
 
UniProt ID:P00520
 
Concentration:0.1mg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
 
Purity:≥70% (SDS-PAGE)
 
Specific Activity:820 nmol/min/mg using a peptide substrate, EAIYAAPFAKKK, in 25mM MOPS, pH 7.2, containing 12.5mM β-glycerol-phosphate, 25mM MgCl2, 5mM EGTA, 2mM EDTA. Add 0.25mM DTT to assay buffer prior to use.
 
Application Notes:Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in cell differentiation, cell division, cell adhesion, and stress responses. The c-Abl protein is inhibited by its SH3 domain, and deletion of this domain turns ABL1 into an oncogene. A chromosomal translocation results in the head-to-tail fusion of the BCR and ABL1 genes. This translocation is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of Abl1 is regulated by CDC2-mediated phosphorylation.
 

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