Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Abl1 (human), (recombinant) (His-tag)

 
BML-SE562-0005 5 µg 249.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Abelson murine leukemia viral oncogene homolog 1
 
Sequence:Human recombinant Abl1 (aa 27-1130) with N-terminal histidine tag.
 
MW:~135kDa
 
Source:Produced in insect cells. Produced in a baculovirus expression system.
 
UniProt ID:P00519
 
Formulation:Liquid. In 50mM sodium phosphate, pH 7.0, containing 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
 
Purity:≥70% (SDS-PAGE)
 
Specific Activity:>700 nmol/min/mg using a peptide substrate, EAIYAAPFAKKK, in 25mM MOPS, pH 7.2, containing 12.5mM β-glycerol-phosphate, 25mM MgCl2, 5mM EGTA, 2mM EDTA. Add 0.25mM DTT to assay buffer prior to use.
 
Application Notes:Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in cell differentiation, cell division, cell adhesion, and stress responses. The c-Abl protein is inhibited by its SH3 domain, and deletion of this domain turns ABL1 into an oncogene. A chromosomal translocation results in the head-to-tail fusion of the BCR and ABL1 genes. This translocation is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of Abl1 is regulated by CDC2-mediated phosphorylation.
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Abl
By biological activity:
Abl Recombinant protein
Abl Enzyme
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,