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PRL-1 (human), (recombinant) (His-tag)

 
BML-SE556-0050 50 µg 431.00 USD
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Product Specification

Alternative Name:Phosphatase of regenerating liver 1, Protein tyrosine phosphatase type IVA 1
 
MW:~19.8kDa
 
Source:Produced in E. coli. The catalytic domain of PRL-1 (aa 2-173) is fused at the N-terminus to a His-tag.
 
EC:3.1.3.48
 
UniProt ID:Q93096
 
Concentration:0.05μg/μl
 
Formulation:Liquid. In 25mM TRIS-HCl, pH 8.0, containing 100mM sodium chloride, 0.05% Tween 20, 20% glycerol and 3mM dithiothreitol.
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:0.168U/μg. One unit will hydrolyze 1pmol para-nitrophenyl phosphate (PNPP) per minute at 37°C. Assay buffer: 50mM TRIS, pH 7.4, containing 150mM sodium chloride, 5mM dithiothreitol and 12.5mM PNPP.
 
Application Notes:Useful for studies of enzyme kinetics and regulation, dephosphorylation of target substrates, and inhibitor screening.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Dilution of the enzyme followed by refreezing may lead to loss of activity.
 
Scientific Background:PRL phosphatases comprise a class of small oncogenic phosphatases that are prenylated at their carboxyl-termini. PRL-1 is overexpressed during cell proliferation in the liver and during differentiation of epithelial cells in the digestive tract. It augments MMP-2 and MMP-9 expression, which in turn induce cell migration and invasion.
 

General Literature References

PRL1 Promotes Cell Migration and Invasion by Increasing MMP2 and MMP9 Expression through Src and ERK1/2 Pathways (dagger): Y. Luo, et al.; Biochemistry 48, 1838 (2009), Abstract;
Expression of PRL-1 nuclear PTPase is associated with proliferation in liver but with differentiation in intestine: R.H. Diamond, et al.; Am. J. Physiol. 271, G121 (1996), Abstract;
PRL-1, a unique nuclear protein tyrosine phosphatase, affects cell growth: R.H. Diamond, et al.; Mol. Cell. Biol. 14, 3752 (1994), Abstract; Full Text

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