Cathepsin K (human), (recombinant)

Highly active



BML-SE553-0010	10 µg	316.00 USD

Cathepsin K, a member of the papain family of cysteine proteases, acts upon proteins such as collagen, AL amyloid, kinin, and elastin. It is a lysosomal protease expressed primarily in osteoclasts, but also in other cell types such as macrophages. It functions in bone remodeling and is implicated in disease states such as osteoporosis, atherosclerosis, arthritis, and pycnodysostosis.

Product Specification

Alternative Name:	Cathepsin O, Cathepsin O2

Concentration:	0.023mg/ml

MW:	~26kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In 50mM sodium acetate, pH 5.5, containing 50mM sodium chloride, 0.5mM EDTA and 5mM DTT.

Specific Activity:	≥1’500mU/mg protein.One unit is defined as the amount of enzyme that will hydrolyze 1µmol Z-Phe-Arg-AMC substrate (OmniCathepsin™ Fluorogenic Substrate, Prod. No. BML-P139) per min. at 37°C in 50mM sodium acetate, pH 5.5, containing 2.5mM EDTA, 0.01% Trition X-100 and 1mM DTT.

EC:	3.4.22.38

Source/Host:	Produced in insect cells. Purified as full-length proenzyme (see Prod. No. BML-SE367), then auto-activated at low pH.

Long Term Storage:	-80°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -80°C.

Handling:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. Keep on dry ice.

Background / Technical Information:	UniProt ID P43235: Cathepsin K (human)

SDS-PAGE Analysis. Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg Cathepsin K

Activation of ProCathepsin K. Lanes 2-7 each contain 1 µg of total protein during time-course activation at low pH. Lane 2: pre-activation, Lane 3: 0 min, Lane 4: 1 hr, Lane 5: 2 hr, Lane 6: 3 hr, Lane 7: 4 hr.

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General Literature References

Cathepsin K-dependent toll-like receptor 9 signaling revealed in experimental arthritis: M. Asagiri, et al.; Science 319, 624 (2008), Abstract;

Cathepsin K: a cysteine protease with unique kinin-degrading properties: E. Godat, et al.; Biochem. J. 383, 501 (2004), Abstract; Full Text

Cathepsin V, a novel and potent elastolytic activity expressed in activated macrophages: Y. Yasuda, et al.; J. Biol. Chem. 279, 36761 (2004), Abstract; Full Text

Cleavage of AL amyloid proteins and AL amyloid deposits by cathepsins B, K, and L: S. Bohne, et al.; J. Pathol. 203, 528 (2004), Abstract;

Cathepsin K--a marker of macrophage differentiation?: F. Bühling, et al.; J. Pathol. 195, 375 (2001), Abstract;

The collagenolytic activity of cathepsin K is unique among mammalian proteinases: P. Garnero, et al.; J. Biol. Chem. 273, 32347 (1998), Abstract; Full Text

Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts: F.H. Drake, et al.; J. Biol. Chem. 271, 12511 (1996), Abstract; Full Text

Pycnodysostosis, a lysosomal disease caused by cathepsin K deficiency: B.D. Gelb, et al.; Science 273, 1236 (1996), Abstract;

The baculovirus cysteine protease has a cathepsin B-like S2-subsite specificity: D. Brömme & K. Okamoto; Biol. Chem. Hoppe Seyler 376, 611 (1995), Abstract;