United States
LSD1 (human), (recombinant)
Highly active
| BML-SE544-0050 | 50 µg | 359.00 USD |  |
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Product Specification
| Alternative Name: | KDM1, Lysine-specific histone demethylase 1, BHC110, AOF2, KIAA0601 |
| MW: | 78 kDa |
| Purity: | ≥90% (SDS-PAGE) |
| Sequence: | N-terminal truncation of LSD1 from human cDNA. Sequence is identical to Genbank accession NM015013 (aa 151-852). |
| Formulation: | Liquid. Solution containing 6mM Na2HPO4, 1.1mM K2HPO4, pH 7.2, 82mM sodium chloride, 1.6mM KCl and 40% (v/v) glycerol. |
| Activity: | Highly active in a peroxidase-coupled assay with the Histone H3 Dimethyl Lysine-4 Peptide (Prod. No. BML-P256). |
| Specific Activity: | >20 U/µg. One U=1 pmol/min at 30°C, 100 µM Histone H3 Dimethyl Lysine-4 Peptide (Prod. No. BML-P256). |
| Source/Host: | Produced in E. coli. |
| Application: | Study LSD1 kinetics and inhibitor sensitivity and also the effect of the enzyme in transcriptional regulation, cell cycle progression, and oncogenesis. |
| Long Term Storage: | -80°C |
| Handling: | After opening, prepare aliquots and store at -80°C. Do not freeze/thaw. |
| Miscellaneous/General: | The mono, di- and trimethylation of particular lysine residues in histone tails (e.g. histone H3 lysine-4 (H3K4), H3K9, H3K27, H3K36, H4K20) are implicated, along with a variety of other post-translational modifications (e.g. lysine acetylation) in the transmission of heritable epigenetic information and the control of chromatin structure and DNA transcription. LSD1 catalyzes the oxidative demethylation of mono- and dimethylated H3K4, producing hydrogen peroxide and formaldehyde in the process. H3K4 methylation is considered a transcription activating chromatin mark and in vivo LSD1 is frequently found in association with the transcriptional corepressor protein CoREST and HDACs 1 or 2. LSD1 is inhibited by a number of established monoamine oxidase inhibitor drugs. That and the fact that its expression is elevated in a number of cancers may make it a promising target for drug development. |
| Background / Technical Information: | Please click here for the comprehensive product datasheet. |
General Literature References
LSD1: oxidative chemistry for multifaceted functions in chromatin regulation.: F. Forneris, et al.; Trends Biochem. Sci. 33, 181 (2008), Abstract;
Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications.: M. Lee, et al.; Chem. Biol. 13, 563 (2006), Abstract;
Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.: F. Forneris, et al.; FEBS Lett. 579, 2203 (2005), Abstract;
Human histone demethylase LSD1 reads the histone code.: F. Forneris, et al.; J. Biol. Chem. 280, 41360 (2005), Abstract;
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.: Y. Shi, et al.; Cell 119, 941 (2004), Abstract;
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