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Erk1 (human), (recombinant) (His-tag)

 
BML-SE511-0020 20 µg 395.00 USD
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Product Specification

Alternative Name:MAPK3, MAP kinase 3
 
MW:~43kDa
 
Source:Produced in insect cells. Active full length Erk1 is fused to an N-terminal His-tag.
 
UniProt ID:P27361
 
Gene/Protein Identifier:NM_002746 (RefSeq)
 
Formulation:Liquid. In 20mM TRIS-HCl, pH 8.0, containing 200mM sodium chloride, 5mM DTT, 0.1% Tween 20, 10% glycerol.
 
Purity:≥85% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Specific Activity:≥5 pmol/µg/min. Kinase activity was measured using the non-radioactive ADP Hunter Plus kit™.
 
Application Notes:Useful for the study of enzyme regulation and kinetics, phosphorylation of target substrates, inhibitor screening.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:After opening aliquot contents and freeze quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen. Avoid repeated freeze/defrost cycles.
 
Scientific Background:A member of the MAP kinase family, ERK1 is ubiquitously expressed and phosphorylates a large number of proteins in all parts of the cell.
 
BML-SE511 SDS-PAGE
SDS-PAGE Analysis: Lane 1: MWM; Lanes 2-5 purified Erk1 Protein (Prod. No. BML-SE511) Lane 2: 0.5 µg; Lane 3: 1.0 µg; Lane 4: 2.0 µg; Lane 5: 5.0 µg
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BML-SE511 SDS-PAGE

General Literature References

Differential regulation and properties of MAPKs.: M. Raman et al.; Oncogene 26, 3100 (2007),
The ERK1/2 mitogen-activated protein kinase pathway as a master regulator of the G1- to S-phase transition.: S. Meloche & J. Pouysségur; Oncogene 26, 3227 (2007),

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