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Proteinase 3 (human neutrophils)

Highly active and high purity serine protease.
 
BML-SE498-0025 25 µg 247.00 USD
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Product Specification

Alternative Name:PR3, Myeloblastin, Wegener's autoantigen
 
MW:~29kDa
 
Source:Isolated from human sputum neutrophils.
 
UniProt ID:P24158
 
Formulation:Liquid. In 0.05M sodium acetate, pH 5, containing 0.6M sodium chloride.
 
Purity:≥95% (SDS-PAGE). Contains no elastase or cathepsin G.
 
Specific Activity:50 U/mg, where one unit hydrolyzes 1μmole of 250μM Boc-Ala-Ala-Nva-SBzl per minute at 25°C in 100mM MOPS pH 7.5, 500mM NaCl, 10% DMSO, 100μM DTNB.
 
Application Notes:Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Although this is in highly purified form, and the source material tested negative for syphilis, HIV-1/HIV-2, Hepatitis B, Hepatitis C, and HTLV-1, handle this product with all due precautions.
 
Scientific Background:Proteinase 3 is a neutral serine protease located in neutrophil azurophil granules. It is closely related to neutrophil elastase and can cleave elastin. PR3 is involved in diseases such as Wegener’s granulomatosis, emphysema, vasculitis, and other inflammatory diseases.
 

Product Literature References

Neutrophil proteinase 3 (PR3) acts on protease-activated receptor-2 (PAR-2) to enhance vascular endothelial cell barrier function: C.J. Kuckleburg, et al.; Arterioscler. Thromb. Vasc. Biol. 33, 275 (2013), Application(s): Cell Culture, Abstract; Full Text
Proteinase 3 reactive T cells in patients with granulomatosis with polyangiitis: S. Scollard, et al.; La Presse Médicale 42, 716 (2013), (Poster), Application(s): Cell Culture, Full Text

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