14-3-3θ/τ (human), (recombinant) (His-tag)



BML-SE487-0100	100 µg	237.00 USD

Product Specification

MW:	~30 kDa

Purity:	>90% (SDS-PAGE)

Formulation:	Liquid. In 50mM NaH₂PO₄ containing 300mM sodium chloride, pH 8.0.

Source/Host:	Produced in E. coli. Human 14-3-3τ/θ is fused at the C-terminus to a His-tag.

Application:	Drug screening, protein-protein interaction studies, gel overlays.

Long Term Storage:	-80°C

Handling:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	14-3-3 proteins are dimeric phosphoserine/phosphothreonine binding proteins, which have been shown to be involved in the regulation of many crucial processes such as signal transduction, cell-cycle control, apoptosis, protein localization, phosphorylation state and stability, transcription, metabolism, and malignant transformation. 14-3-3θ/t has been shown to be present in testis and 14-3-3θ mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord. Both PKCµ and Bax have been shown to be negatively regulated by 14-3-3θ

Background / Technical Information:	Please click here for the comprehensive product datasheet.

General Literature References

C-TAK1 interacts with microphthalmia-associated transcription factor, Mitf, but not the related family member Tfe3: T. Schwarz, et al.; BBRC 394, 890 (2010), Abstract;

14-3-3 Interacts directly with and negatively regulates pro-apoptotic Bax.: M. Nomura et al.; J Biol Chem 278, 2058 (2003), Abstract;

How do 14-3-3 proteins work? Gatekeeper phosphorylation and the molecular anvil hypothesis.: M.B. Yaffe; FEBS Lett. 513, 53 (2002), Abstract;

A 14-3-3 mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord.: A. Malaspina et al.; J. Neurochem. 75, 2511 (2000), Abstract;

Characterization of a novel transcript of 14-3-3 theta in Sertoli cells.: J. Chaudhary & M.K. Skinner; J. Androl. 21, 730 (2000), Abstract;

Protein kinase C mu is negatively regulated by 14-3-3 signal transduction proteins.: A. Hausser et al.; J. Biol. Chem. 274, 9258 (1999), Abstract;

14-3-3 proteins interact with the insulin-like growth factor receptor but not the insulin receptor.: R.W. Furlanetto et al.; Biochem. J. 327, 765 (1997), Abstract;

Molecular cloning and tissue-specific expression of the mouse homologue of the rat brain 14-3-3 theta protein: characterization of its cellular and developmental pattern of expression in the male germ line.: L. Perego & G. Berruti; Mol. Reprod. Dev. 47, 370 (1997), Abstract;