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MKP-2/Erk1 (human), (recombinant)

 
BML-SE463-0100 100 µg 357.00 USD
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Approximately 1:1 stoichiometric complex of MKP-2 with Erk1.

Product Specification

Alternative Name:MAP kinase phosphatase 2/Erk1, DUSP4/Erk1, Dual specificity protein phosphatase 4/Erk1, HVH2/Erk1
 
MW:42.3 and 43.1 kDa
 
Source:Produced in Sf9 cells.
 
UniProt ID:Q13115 (MKP2), P27361 (Erk1)
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, 200mM NaCl, 5mM DTT, 0.1 % Tween-20, 10% glycerol.
 
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
 
Specific Activity:≥40 U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:MKP-2 (DUSP4) has an N-terminal MAP kinase binding domain. Co-expressing MKP-2 with Erk1 MAP kinase results in increased phosphatase activity compared to MKP-2 expressed alone.
 

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