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MKP-3 (human), (recombinant)

 
BML-SE454-0100 100 µg 387.00 USD
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Product Specification

Alternative Name:MAP kinase phosphatase 3, DUSP6, Dual specificity protein phosphatase 6, PYST1
 
MW:42 kDa
 
Source:Produced in Sf9 cells. Recombinant full length MKP-3.
 
UniProt ID:Q16828
 
Gene/Protein Identifier:NM_001946 (RefSeq)
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, 200mM NaCl, 5mM DTT, 0.1 % Tween-20, 10% glycerol.
 
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
 
Specific Activity:≥10 U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:MKP-3 is a member of a group of dual specificity phosphatases which negatively regulate MAP kinases, dephosphorylating both the phosphotyrosine and phosphothreonine in the pTXpY activation loop motif. Transcriptional suppression of MKP-3 expression has been implicated in the development of pancreatic cancer. MKP-3 is located in the nucleus where it may act to anchor Erk 1/2.
 
BML-SE454 SDS-PAGE
SDS-PAGE Analysis. Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg, Lane 4: 5 µg MKP-3.
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BML-SE454 SDS-PAGE

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