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Cathepsin S (human), (recombinant)

Highly active
BML-SE453-0010 10 µg 388.00 USD
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Product Specification

MW:26 kDa
Source:Produced in insect cells. Recombinant active cathepsin S cloned from human cDNA as full length proenzyme, auto-activated by incubation in pH 4.5 acetate buffer, and purified. Produced in a baculovirus expression system.
UniProt ID:P25774
Formulation:Liquid. In 100 mM MES, pH 6.5, containing 1 mM EDTA, 50 mM L-cysteine, 10 mM DTT, 0.5% Triton® X-100, 30% glycerol.
Purity:≥95% (SDS-PAGE)
Purity Detail:Purified by multi-step chromatography.
Specific Activity:≥20 U/mg protein. One unit will hydrolyze one µmole Z-Val-Val-Arg-AMC substrate (Prod. No. BML-P199, 40 µM) per minute at 37°C.
Application Notes:Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Scientific Background:Cathepsin S, a member of the papain family of lysosomal cysteine proteases, functions in the immune system.
Cathepsin S (human), (recombinant) SDS-PAGE
SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 0.5 μg of Human Cathepsin S Protein
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Cathepsin S (human), (recombinant) SDS-PAGE

Product Literature References

Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.) 50, 10875 (2014), Abstract;
Development of cell-active non-peptidyl inhibitors of cysteine cathepsins: D. Dana, et al.; Bioorg. Med. Chem. 21, 2975 (2013), Abstract;

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BML-P199-0010 10 mg 127.00 USD
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