Product Details
| MW: | 26 kDa |
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| Source: | Produced in insect cells. Recombinant active cathepsin S cloned from human cDNA as full length proenzyme, auto-activated by incubation in pH 4.5 acetate buffer, and purified. Produced in a baculovirus expression system. |
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| UniProt ID: | P25774 |
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| Formulation: | Liquid. In 100 mM MES, pH 6.5, containing 1 mM EDTA, 50 mM L-cysteine, 10 mM DTT, 0.5% Triton® X-100, 30% glycerol. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Specific Activity: | ≥20 U/mg protein. One unit will hydrolyze one µmole Z-Val-Val-Arg-AMC substrate (Prod. No. BML-P199, 40 µM) per minute at 37°C. |
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| Application Notes: | Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Cathepsin S, a member of the papain family of lysosomal cysteine proteases, functions in the immune system. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 0.5 μg of Human Cathepsin S Protein
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Product Literature References
An evolutionary molecular adaptation of an unusual stefin from the liver fluke Fasciola hepatica redefines the cystatin superfamily: M. Busa, et al.; J. Biol. Chem.
299, 102970 (2023),
Abstract;
Two Tags in One Probe: Combining Fluorescence- and Biotin-based Detection of the Trypanosomal Cysteine Protease Rhodesain: C. Lemke, et al.; Chemistry
28, e202201636 (2022),
Abstract;
Cell penetrable, clickable and tagless activity-based probe of human cathepsin L: D. Dana, et al.; Bioorg. Chem.
85, 505 (2019),
Application(s): Activity assay,
Abstract;
Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.)
50, 10875 (2014),
Abstract;
Development of cell-active non-peptidyl inhibitors of cysteine cathepsins: D. Dana, et al.; Bioorg. Med. Chem.
21, 2975 (2013),
Abstract;
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