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MKP-1/p38 complex (human), (recombinant)

 
BML-SE440-0100 100 µg 404.00 USD
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Approximately 1:1 stoichiometric complex of recombinant human MKP-1 (MAP Kinase Phopshatase-1, DUSP1, CL100) with recombinant human p38α.

Product Specification

Alternative Name:MAP kinase phosphatase-1/p38 complex, DUSP1/p38 complex, CL100/p38 complex
 
MW:39/41 kDa
 
Source:Produced in Sf9 insect cells. Produced in a baculovirus expression system.
 
UniProt ID:P28562 (MKP1)
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, 200mM NaCl, 5mM DTT, 0.1 % Tween-20, 10% glycerol.
 
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
 
Specific Activity:≥4 U/µg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:In addition to their C-terminal phosphatase domains, various MAPK phosphatases, including MKP-1, comprise an N-terminal MAPK binding domain. Binding of p38α to MKP-1 mediates its catalytic activation both in vivo and in vitro.
 

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