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DPPIV (human), (recombinant)

Highly active
 
BML-SE434-0025 25 mU 362.00 USD
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Product Specification

Alternative Name:Dipeptidyl Peptidase IV, DPP4, CD26
 
MW:85.4 kDa
 
Source:Produced in Sf9 cells using baculovirus expression system. Active recombinant human DPPIV (aa 29-766). This represents a naturally-occurring cleaved (soluble) form of DPPIV.
 
EC:3.4.14.5
 
UniProt ID:P27487
 
Formulation:Liquid. In 10 mM TRIS, pH 8.0, 100mM NaCl.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Specific Activity:One unit produces 1µmole free pNA per minute, using as substrate 100 µM H-Gly-Pro-pNA (Prod. No. BML-P188) in 50mM TRIS pH 7.5 at 37°C.
 
Application Notes:Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:The enzyme is stable on ice for at least several hours.  However, it is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. After initial defrost, aliquot product into individual tubes and refreeze at -70°C. Avoid repeated freeze/defrost cycles.
NOTE: When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.
 
Scientific Background:DPPIV is involved in diseases such as diabetes (via cleavage of GIP and GLP-1) and cancer.
 
BML-SE434 SDS-PAGE
SDS-PAGE Analysis: Lane 1: MWM; Lane 2: 1.0 μg of purifed Human DPPIV protein.
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BML-SE434 SDS-PAGE

Product Literature References

Pharmacological profiles of gemigliptin (LC15-0444), a novel dipeptidyl peptidase-4 inhibitor, in vitro and in vivo: S. Kim, et al.; Eur. J. Pharmacol. 788, 54 (2016), Application(s): Enzyme Inhibition assay (in vitro), Abstract;

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DPP substrate
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BML-P188-0025 25 mg 80.00 USD
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