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VEGFR2 (human), (recombinant) (GST-tag)

 
BML-SE404-0010 10 µg 375.00 USD
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Product Specification

Alternative Name:KDR, FLK-1, Vascular endothelial growth factor receptor 2
 
MW:~87 kDa
 
Source:Produced in Sf9 cells with N-terminal GST tage. Human VEGFR2 (aa 805-1356).
 
UniProt ID:P35968
 
GenBank ID:AF035121
 
Formulation:Liquid. In 40mM TRIS-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 20% Glycerol, and 3mM DTT.
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:56 U/μg. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of tyrosine substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 μM Naorthovanadate, 0.5 mM ATP, 30 μg/ml Poly (Glu:Tyr)4:1 substrate, and 0.04 ug recombinant VEGFR2. 
 
Application Notes:Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:

When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity.

 
Handling:After opening, prepare aliquots, freeze in liquid nitrogen and store at -80°C. Avoid freeze/thaw cycles.
 

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