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Lyn B (human), (recombinant) (GST-tag)

 
BML-SE385-0005 5 µg 257.00 USD
 
BML-SE385-0020 20 µg 606.00 USD
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Product Specification

Alternative Name:Tyrosine protein kinase Lyn B
 
MW:~85 kDa
 
Source:Produced in insect cells. Full length active enzyme. Produced in a baculovirus expression system.
 
UniProt ID:P07948-2
 
Formulation:Liquid. 0.1 mg/ml in 50 mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:180 nmol/min/mg. Kinase activity was measured as the molar amount of phosphate incorporated into poly (Glu-Tyr) at 30°C using a concentration of 50μM [32P]ATP in 25mM MOPS, pH 7.2, 12.5mM β-glycerophosphate, 5mM EGTA, 2mM EDTA, 25mM MgCl2, 0.25mM DTT (added right before using).
 
Application Notes:Useful for kinetic and functional studies, phosphorylation of target substrates, drug screening.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible.
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Replacement for ADI-PPK-431
 

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