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Src (human), (recombinant) (GST-tag)

 
BML-SE357-0005 5 µg 210.00 USD
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Product Specification

Alternative Name:pp60src
 
MW:~83kDa
 
Source:Produced in E. coli.
 
UniProt ID:P12931
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
 
Purity:≥95% (SDS-PAGE)
 
Application Notes:Useful for kinetic and functional studies, phosphorylation of target substrates, drug screening.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:Src, a non-receptor type tyrosine kinase, is a proto-oncogene that plays key roles in cell morphology, motility, proliferation, and survival. Src is overexpressed and activated in a large number of human cancers. Oncogenic forms of Src alter cell structure and affect thin filaments and adhesion networks that control cell migration. Src also influences the RhoA-ROCK pathway that controls actin polymerization, the STAT family of transcription factors which are involved in tumorigenesis and the Cbl ubiquitin ligase that controls Src protein levels.
 

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