PTP1B (human), (recombinant)



BML-SE332-0050	50 µg	335.00 USD

Product Specification

Alternative Name:	Protein tyrosine phosphatase 1B

Concentration:	0.5mg/ml

MW:	~37.4kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In 50mM HEPES, pH7.2, containing 1mM EDTA, 5mM DTT, and 0.05% NP-40.

Specific Activity:	~50μmol/min/mg at 30°C using 0.15mM of phosphopeptide substrate based on EGFR residues 988-998 (Prod. No. BML-P323) in 50mM HEPES, pH 7.2, 1mM EDTA, 1mM DTT, 0.05% NP-40, and 0.2μg/μl BSA.

Source/Host:	Produced in E. coli. Human PTP1B (protein tyrosine phosphatase 1B) (aa 1-322).

Application:	Useful for the study of tyrosine phosphatase kinetics, substrate specificity and for screening inhibitors.

Long Term Storage:	-80°C

Handling:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	PTP1B is the ubiquitous, prototypical nontransmembrane PTP originally identified in human placenta.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg, Lane 4: 5 µg PTP1B. The higher molecular weight bands are BSA included for stability in storage buffer.

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Product Literature References

Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design: Y.A. Puius, et al.; PNAS 94, 13420 (1997), Abstract;

Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas): F. Liu, et al.; J. Biol. Chem. 271, 31290 (1996), Abstract;