PTP1B (human), (recombinant) - Enzo Life Sciences

BML-SE332

Revised 05-Mar-10

PTP1B (human), (recombinant)
SYNONYMS	Protein tyrosine phosphatase 1B
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Protein Tyrosine Phosphatases Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
BML-SE332-0050		50 µg	325.00 USD

Product Specification

MW:	~37.4kDa
SOURCE/HOST:	Produced in E. coli. Human PTP1B (protein tyrosine phosphatase 1B) (aa 1-322).
CONCENTRATION:	0.5mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM HEPES, pH7.2, containing 1mM EDTA, 5mM DTT, and 0.05% NP-40.
SPECIFIC ACTIVITY:	~50μmol/min/mg at 30°C using 0.15mM of phosphopeptide substrate based on EGFR residues 988-998 (Prod. No. BML-P323) in 50mM HEPES, pH 7.2, 1mM EDTA, 1mM DTT, 0.05% NP-40, and 0.2μg/μl BSA.
APPLICATION:	Useful for the study of tyrosine phosphatase kinetics, substrate specificity and for screening inhibitors.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Product Specific Literature References

Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas): F. Liu, et al.; J. Biol. Chem. 271, 31290 (1996) Abstract
Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design: Y.A. Puius, et al.; PNAS 94, 13420 (1997) Abstract

General Information

PTP1B is the ubiquitous, prototypical nontransmembrane PTP originally identified in human placenta.
BACKGROUND/TECHNICAL INFORMATION
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