Replaces Prod. #: ALX-200-005
Product Details
| Alternative Name: | CaM, Phosphodiesterase 3':5'-cyclic nucleotide activator |
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| Sequence: | Sequence identical to Genbank accession M27319.1. |
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| MW: | 16.8 kDa |
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| Source: | Produced in E. coli. |
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| CAS: | 73298-54-1 |
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| MI: | 14: 1719 |
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| UniProt ID: | P62158 |
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| Formulation: | Lyophilized. Salt-free. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Biological Activity: | Elicits Ca2+-dependent activation of calcineurin (Ca2+/calmodulin-dependent Ser/Thr protein phosphatase 2B, PP-2B) with the same potency and to the same maximum extent as purified native calmodulin. |
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| Application Notes: | Calmodulin can be used to activate calmodulin-dependent phosphodiesterase, calcineurin, CaM kinases, etc. |
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| Reconstitution: | Reconstitute in water or an appropriate buffer. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Human recombinant calmodulin (Prod. No. BML-SE325) activation of human calcineurin is essentially identical to that of purified bovine brain calmodulin (native, bovine). Initial rates of calcineurin phosphatase activity were determined as a function of calmodulin concentration with the Calcineurin Colorimetric Drug Discovery Kit (Prod. No. BML-AK804). (Note that the human and bovine calmodulin amino acid sequences are 100% identical.)
SDS-PAGE Analysis: Lane 1: MW Marker; Lane 2: 1.0 µg of purified human recombinant calmodulin protein (Prod. No. BML-SE325).
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Product Literature References
NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly: T. Niu, et al.; Nat. Commun.
12, 5862 (2021),
Abstract;
Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast: R. Martin-Garcia, et al.; Cell Rep.
25, 772 (2018),
Abstract;
A model for regulation by SynGAP-α1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density: W.G. Walkup, et al.; Elife
5, e22495 (2016),
Application(s): Measurement of affinity of CaM for r-synGAP-a1 by SPR,
Abstract;
Full Text
The phosphatase calcineurin regulates pathological TDP-43 phosphorylation: N.F. Liachko, et al.; Acta Neuropathol.
4, 545 (2016),
Abstract;
Dissecting cooperative communications in a protein with a high-throughput single-molecule scalpel: Z. Yu, et al.; Chemphyschem.
16, 223 (2015),
Abstract;
