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14-3-3σ (human), (recombinant) (GST-tag)

 
BML-SE316-0100 100 µg 284.00 USD
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Product Specification

MW:~60 kDa
 
Source:Produced in E. coli. Human 14-3-3σ is fused at the N-terminus to a GST-tag.
 
UniProt ID:P31947
 
Formulation:Liquid. In 50mM TRIS/HCl, pH 7.5, 150mM NaCl.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Application Notes:Can be used for protein-protein interaction studies and gel overlays.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:14-3-3 proteins are dimeric phosphoserine/phosphothreonine binding proteins, which have been shown to be involved in the regulation of many crucial processes such as signal transduction, cell-cycle control, apoptosis, protein localization, phosphorylation-state and stability, transcription, metabolism, and malignant transformation. 14-3-3σ is the main 14-3-3 isoform that has been directly implicated in the development of cancer. The 14-3-3σ gene is directly regulated by p53 and is silenced by CpG methylation in a large number of carcinomas.
 
BML-SE316 SDS-PAGE
SDS-PAGE Analysis: Lane1: MWM; Lane 2: 1.0 μg of purified human 14-3-3σ protein.
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BML-SE316 SDS-PAGE

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