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Neutrophil elastase (human), (purified)

 
BML-SE284-0100 100 µg 308.00 USD
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Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1- antichymotrypsin. Because of its potential roles in inflammatory diseases such as emphysema and cystic fibrosis, it is a target for drug discovery.

Product Specification

Alternative Name:Leukocyte elastase, LE
 
Source:Isolated from human neutrophils. Active enzyme.
 
UniProt ID:P08246
 
Purity:≥95% (SDS-PAGE). Although this is in highly purified form, and the source material tested negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2, handle this product with all due precautions.
 
Specific Activity:20-22 U/mg protein. One unit will hydrolyze 1.0 µmole of MeOSuc-Ala-Ala-Pro-Val-pNA (Prod. No. BML-P213) per minute, at 25°C, pH 8.0.
 
Application Notes:Useful for inhibitor screening, kinetic studies, and cleavage of substrates in vitro or in tissue culture.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:This enzyme is stable when stored under the above conditions. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:Neutrophil elastase, a serine protease found in polymorphonuclear neutrophils (PMNs), functions in inflammation.
 

Product Literature References

Calcium-induced Tetramerization and Zinc Chelation Shield Human Calprotectin fromDegradation by Host and Bacterial Extracellular Proteases: J.R. Stephan & E.M. Nolan; Chem. Sci. 2016, 1962 (2016), Application(s): Protease digestion assays, Abstract; Full Text

General Literature References

3-Acylamino-azetidin-2-one as a novel class of cysteine proteases inhibitors: N.E. Zhou, et al.; Bioorg. Med. Chem. Lett. 13, 139 (2003), Abstract;
6-Acylamino-2-1(ethylsulfonyl)oxy]-1H-isoindole-1,3-diones mechanism-based inhibitors of human leukocyte elastase and cathepsin G: effect of chirality in the 6-acylamino substituent on inhibitory potency and selectivity: L.M. Vagnoni, et al.; Bioorg. Med. Chem. 9, 637 (2001), Abstract;
Inhibition of serine proteases by functionalized sulfonamides coupled to the 1,2,5-thiadiazolidin-3-one 1,1 dioxide scaffold: W.C. Groutas, et al.; Bioorg. Med. Chem. 9, 1543 (2001), Abstract;
Human coagulation factor V is activated to the functional cofactor by elastase and cathepsin G expressed at the monocyte surface: D.H. Allen & P.B. Tracy; J. Biol. Chem. 270, 1408 (1995), Abstract;
Inactivation of recombinant human tumor necrosis factor-alpha by proteolytic enzymes released from stimulated human neutrophils: K.P. van Kessel, et al.; J. Immunol. 147, 3862 (1991), Abstract;
Biology of the Extracellular Matrix, vol. 1: Regulation of Matrix Accumulation: J.G. Bieth; New York: Academic Press (R.P. Mecham, ed.) 217-320 (1986), Book,
Human leukocyte granule elastase: rapid isolation and characterization: R.J. Baugh and J. Travis; Biochemistry 15, 836 (1976), Abstract;

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Substrate for neutrophil elastase and neutrophil proteinase 3
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BML-P213-0005 5 mg 85.00 USD
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