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MMP-11 (catalytic domain) (human), (recombinant)

 
BML-SE282-0010 10 µg 318.00 USD
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Product Specification

Alternative Name:Matrix metalloproteinase 11, Stromelysin-3
 
MW:19.3 kDa
 
Source:Produced in E. coli. Active Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-11 (Phe98-Ser266, NM_005940) with a C-terminal purification tag. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. It may be an important link between obesity and cancer.
 
UniProt ID:P24347
 
Formulation:Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20µM ZnCl2, 0.5% Brij-35, and 30% glycerol.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Specific Activity:Due to its unusual substrate preferences [A(A/Q)(N/A)~(L/Y)(T/V/M/R)(R/K), or G(G/A)E~LR5], MMP-11 cleaves MMP peptide substrates such as Prod. No. BML-P125, BML-P126, and BML-P132 extremely slowly (several hours yield very little product). Therefore, the activity of each lot of MMP-11 is verified by digestion of macromolecules.
 
Application Notes:Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:MMP-11 may be a link between obesity and cancer.
 
BML-SE282 SDS-PAGE
Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 μg α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 μg MMP-11, (0 hours); 5. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (0 hours); 10. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (24 hours); 11. 1.5 μg α1-antitrypsin (24 hours); 12. Marker.
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BML-SE282 SDS-PAGE

Product Literature References

Cancer cells, adipocytes and matrix metalloproteinase 11: a vicious tumor progression cycle: E.R. Motrescu & M.C. Rio; Biol. Chem. 389, 1037 (2008), Abstract;

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