Product Details
| Alternative Name: | Sirtuin 3 |
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| MW: | 32.7 kDa |
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| Source: | Produced in E. coli. Active SIRT3 (aa 102-399). Contains a N-terminal His-tag. |
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| UniProt ID: | Q9NTG7 |
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| Formulation: | Liquid. In 25mM TRIS, pH 7.5, containing 100mM NaCl, 5mM DTT, and 10% glycerol. |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Specific Activity: | One unit will deacetylate 1pmol/min of FLUOR DE LYS®-SIRT2 substrate (Prod. No. BML-KI179) at 37°C , using 500µM FLUOR DE LYS®-SIRT2 substrate, 500µM NAD+. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Sirtuins, which are NAD-dependent lysine deacetylases, have been implicated in the control of longevity in yeast and C. elegans and, in yeast, have been shown to be necessary for the longevity increase effected by caloric restriction. Human SIRT3, one of seven human sirtuins, has been assigned, along with SIRT1 and SIRT2, to the class I sirtuin homology group. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem.
62, 5844 (2019),
Abstract;
Pharmacophore modeling and virtual screening studies to identify novel selective SIRT2 inhibitors: G. Eren, et al.; J. Mol. Graph. Model.
10, 1313 (2019),
Abstract;
Aging Promotes Sirtuin 3-Dependent Cartilage Superoxide Dismutase 2 Acetylation and Osteoarthritis: Y. Fu, et al.; Arthritis Rheumatol.
68, 1887 (2016),
Abstract;
Full Text
SIRT3 Deacetylates Ceramide Synthases: Implications for Mitochondrial Dysfunction and Brain Injury: S.A. Novgorodov, et al.; J. Biol. Chem.
291, 1957 (2016),
Application(s): Deacetylation assay with mouse brain cells,
Abstract;
Full Text
A mechanism-based potent sirtuin inhibitor containing Nε-thiocarbamoyl-lysine (TuAcK): B.M. Hirsch, et al.; Bioorg. Med. Chem. Lett.
21, 4753 (2011),
Abstract;
Full Text
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