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MMP-14 (catalytic domain) (human), (recombinant)

 
BML-SE259-0010 10 µg 384.00 USD
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Product Specification

Alternative Name:Matrix metalloproteinase 14, Membrane type matrix metalloproteinase 1, MT-1 MMP
 
MW:22.5 kDa
 
Source:Produced in E.coli. Active Matrix Metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase 1, MT1-MMP) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-14 (Tyr112-Arg298, NM_004995) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-14 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected.
 
UniProt ID:P45452
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 5mM calcium chloride, 300mM sodium chloride, 20µM zinc chloride, 0.5% Brij-35, and 30% glycerol.
 
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
 
Activity:Preincubation of MMP-14 catalytic domain at 13.6nM with the broad-spectrum inhibitor GM6001 (Prod. No. BML-EI300) at 20nM for 1 hour completely inhibits enzymatic activity.
 
Specific Activity:≥70 U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 µM; Prod. No. BML-P125) as substrate.
 
Application Notes:Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:The enzyme is stable on ice for at least several hours. However, it is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. NOTE: When stored at -70°C, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.
 
Handling:After opening, prepare aliquots and store at -70°C. Avoid freeze/thaw cycles.
 

Product Literature References

Regulation of ATPase activity of transglutaminase 2 by MT1-MMP: implications for mineralization of MC3T3-E1 osteoblast cultures: Y. Nakano, et al.; J. Cell. Physiol. 223, 260 (2010), Abstract;
The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interacts with membrane type 1 matrix metalloproteinase and CD13/aminopeptidase N and modulates their endocytic pathways: T. Miki, et al.; J. Biol. Chem. 282, 12341 (2007), Abstract; Full Text

Related Products

GM-6001 

MMP inhibitor
142880-36-2, ≥98% (HPLC) | Print as PDF
 
BML-EI300-0001 1 mg 68.00 USD
 
BML-EI300-0005 5 mg 269.00 USD
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MMP substrate (chromogenic) 

Sensitive chromogenic MMP substrate
≥97% (HPLC) | Print as PDF
 
BML-P125-0005 5 mg 197.00 USD
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