Product Details
| Alternative Name: | Sirtuin 1 |
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| MW: | 82 kDa |
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| Source: | Produced in E. coli. Recombinant SIRT1 from human cDNA containing an N-terminal His-tag. |
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| UniProt ID: | Q96EB6 |
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| Gene/Protein Identifier: | NM_012238 (RefSeq) |
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| Formulation: | Liquid. In 25 mM TRIS, pH 7.5, 100mM NaCl, 5mM DTT and 10% glycerol. |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Specific Activity: | One unit = 1pmol/min at 37°C, 250µM FLUOR DE LYS® substrate (Prod. No. BML-KI104), 500µM NAD+. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem.
62, 5844 (2019),
Abstract;
Pharmacophore modeling and virtual screening studies to identify novel selective SIRT2 inhibitors: G. Eren, et al.; J. Mol. Graph. Model.
10, 1313 (2019),
Abstract;
Two Novel Proline-Containing Catechin Glucoside from Water-Soluble Extract of Codonopsis pilosula: F.Y. Qin, et al.; Molecules
23, E180 (2018),
Application(s):SIRT1 Inhibition,
Abstract;
Full Text
Design, synthesis and structure-activity relationship studies of novel sirtuin 2 (SIRT2) inhibitors with a benzamide skeleton: T. Sakai, et al.; Bioorg. Med. Chem.
23, 328 (2015),
Abstract;
Discovery of bicyclic pyrazoles as class III histone deacetylase SIRT1 and SIRT2 inhibitors: E. Therrien, et al.; Bioorg. Med. Chem. Lett.
25, 2514 (2015),
Abstract;
Functionalized tetrahydro-1H-pyrido[4,3-b]indoles: a novel chemotype with Sirtuin 2 inhibitory activity: T. Yang, et al.; Eur. J. Med. Chem.
92, 145 (2015),
Abstract;
AROS has a context-dependent effect on SIRT1: T. Kokkola, et al.; FEBS Lett.
588, 1523 (2014),
Application(s): In vitro activity assay,
Abstract;
Cytotoxicity and cell death mechanisms induced by a novel bisnaphthalimidopropyl derivative against the NCI-H460 non-small lung cancer cell line: R.T. Lima, et al.; Anticancer Agents Med. Chem.
13, 414 (2013),
Abstract;
Redox Factor-1 Activates Endothelial SIRTUIN1 through Reduction of Conserved Cysteine Sulfhydryls in Its Deacetylase Domain: S.B. Jung, et al.; PLoS One
8, e65415 (2013),
Abstract;
Full Text
Regulation of inositol 1,3,4-trisphosphate 5/6-kinase (ITPK1) by reversible lysine acetylation: C. Zhang, et al.; Proc. Natl. Acad. Sci U.S.A.
109, 2290 (2012),
Abstract;
Full Text
Role of deleted in breast cancer 1 (DBC1) protein in SIRT1 deacetylase activation induced by protein kinase A and AMP-activated protein kinase: V. Nin, et al.; J. Biol. Chem.
287, 23489 (2012),
Abstract;
Full Text
A brain-permeable small molecule reduces neuronal cholesterol by inhibiting activity of sirtuin 2 deacetylase: D.M. Taylor, et al.; ACS Chem. Biol.
6, 540 (2011),
Abstract;
Class IIa histone deacetylases are hormone-activated regulators of FOXO and mammalian glucose homeostasis: M.M. Mihaylova, et al.; Cell
145, 607 (2011),
Abstract;
Full Text
Electrophoretically mediated microanalysis assay for sirtuin enzymes: Y. Fan, et al.; Electrophoresis
23, 3874 (2010),
Abstract;
SIRT1 is a redox-sensitive deacetylase that is post-translationally modified by oxidants and carbonyl stress: S. Caito, et al.; FASEB J.
24, 3145 (2010),
Application(s): Mass spectrometry analysis of SIRT1 modified by reactive aldehydes,
Abstract;
Full Text
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