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Nupherin™ Transfection Reagent

Greatly enhances transfection efficiency
 
BML-SE225-0075 0.75 mg 138.00 USD
 
BML-SE225-0003 3 mg 479.00 USD
Do you need bulk/larger quantities?
 
  • Dramatically enhances performance of commercially available cationic lipids
  • Allows high-efficiency lipofection (10-40%) of neuronal and ganglion cells
  • Fully compatible with cells grown on glass coverslips, plastic plates or tissue-culture flasks  
Nupherin™ dramatically enhances the performance of commercially available cationic lipids to allow high-efficiency lipofection (10-40%) of neuronal, ganglion and other difficult to transfect cells. Efficient lipofection is limited by the ability of transfected DNA to enter the nucleus – a particular obstacle in nondividing or slow dividing cells like neurons. Nupherin, a peptide based reagent that combines a non-classical nuclear localization sequence (M9) with a nucleotide binding domain, helps to transport DNA into the transfected cell's nucleus. Nupherin has been demonstrated to provide high efficiency lipofection of primary rat hippocampal neurons, chicken embryonic retinal ganglian cells, rat pheochromocytoma (PC12) cells and other recalcitrant cells. Fully compatible with cells grown on glass coverslips, in plastic plates, or in tissue-culture flasks.

Product Specification

Quantity:The 3 mg size provides enough reagent for 100-200 transfections.
 
Concentration:3mg/ml
 
Formulation:Liquid. Sterile solution.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:On first use, aliquot the reagent and store at -80°C. Thawed aliquots of Nupherin™ can be frozen after use for long term storage or refrigerated for up to 1 week. Do not dilute reagent.
 
Handling:Avoid freeze/thaw cycles.
 
bml-se225
Figures: A. Chicken Embryonic Retinal Ganglia, B. Primary neuron lipofection, C. Embryonic rat primary hippocampal neuron
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Product Literature References

Docetaxel induces Bcl-2- and pro-apoptotic caspase-independent death of human prostate cancer DU145 cells: T. Ogura, et al.; Int. J. Oncol. 48, 2330 (2016), Application(s): Tranfection of human prostate cancer cell line, Abstract; Full Text
Docetaxel induces Bcl-2- and pro-apoptotic caspase-independent death of human prostate cancer DU145 cells: T. Ogura, et al.; Int. J. Oncol. 48, 2330 (2016), Application(s): Tranfection of human prostate cancer cell line, Abstract; Full Text
Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation: M. Antkowiak, et al.; Sci. Rep. 3, 3281 (2013), Application(s): Optical transfection of Embryonic rat cortical neurons (E18), Abstract; Full Text
HSF1 transcriptional activity mediates alcohol induction of Vamp2 expression and GABA release: F.P. Varodayan, et al.; Front. Integr. Neurosci. 7, 89 (2013), Application(s): Transfection of cortical neurons, Abstract; Full Text
Focal adhesion kinase regulates neuronal growth, synaptic plasticity and hippocampus-dependent spacial learning and memory: F.J. Monje, et al.; Neurosignals 20, 1 (2012), Application(s): Enhancement of transfection of hippocampal neurons, Abstract; Full Text
Gemfibrozil, a lipid-lowering drug, upregulates IL-1 receptor antagonist in mouse cortical neurons: implications for neuronal self-defense: G.T. Corbett, et al.; J. Immunol. 189, 1002 (2012), Application(s): Transfection of primary neurons, Abstract; Full Text
Pharmacological Chaperoning of Nicotinic Acetylcholine Receptors Reduces the Endoplasmic Reticulum Stress Response: R. Srinivasan, et al.; Mol. Pharmacol. 193, 63 (2012), Application(s): Transfection of mouse Neuro-2a cells, Abstract; Full Text
Alcohol induces synaptotagmin 1 expression in neurons via activation of heat shock factor 1: F.P. Varodayan, et al.; Neuroscience 193, 63 (2011), Application(s): Transfection of cortical neurons, Abstract; Full Text
Enhancement and optimization of plasmid transfection in femtosecond optical transfection: B.B. Praveen, et al.; J. Biophotonics 4, 229 (2011), Application(s): Enhancement of optical transfection efficiency, Abstract;
An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy: B.S. Khakh, et al.; J. Neurosci. 25, 6911 (2005), Abstract; Full Text
Rat embryonic motoneurons in long-term co-culture with Schwann cells - a system to investigate motoneuron diseases on a cellular level in vitro: K. Haastert, et al.; J. Neurosci. Methods 142, 275 (2005), Abstract;
Thioredoxin is downstream of Smad7 in a pathway that promotes growth and suppresses cisplatin-induced apoptosis in pancreatic cancer: N.B. Arnold, et al.; Cancer Res. 64, 3599 (2004), Abstract; Full Text
Vanilloid receptor 1 regulates multiple calcium compartments and contributes to Ca2+-induced Ca2+ release in sensory neurons: L.J. Karai, et al.; J. Biol. Chem. 279, 16377 (2004), Abstract; Full Text
An in vitro study of the sub-cellular distribution of nicotinic receptors: A. Devillers-Thiery, et al.; Biol. Cell. 95, 373 (2003), Abstract; Full Text
Assembly of alpha4beta2 nicotinic acetylcholine receptors assessed with functional fluorescently labeled subunits: effects of localization, trafficking, and nicotine-induced upregulation in clonal mammalian cells and in cultured midbrain neurons: R. Nashmi, et al.; J. Neurosci. 23, 11554 (2003), Abstract; Full Text
Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels: E.M. Slimko & H.A. Lester; J. Neurosci. Methods 124, 75 (2003), Abstract;
Microtubule destabilization and nuclear entry are sequential steps leading to toxicity in Huntington’s disease: E. Trushina, et al.; PNAS 100, 12171 (2003), Abstract; Full Text
The kinesin KIF17b and RNA-binding protein TB-RBP transport specific cAMP-responsive element modulator-regulated mRNAs in male germ cells: V. Chennathukuzhi, et al.; PNAS 100, 15566 (2003), Application(s): Nupherin increses FuGENE 6 transfection in rat embryonic hippocampal neurons, Abstract; Full Text
Selective electrical silencing of mammalian neurons in vitro by the use of invertebrate ligand-gated chloride channels: E.M. Slimko, et al.; J. Neurosci. 22, 7373 (2002), Abstract; Full Text

General Literature References

Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells: A. Subramanian et al.; Nat. Biotechnol. 17, 873 (1999), Abstract;

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