Ubiquitin-AMC

High purity industry standard substrate for deubiquitinylating enzymes (DUBs).



BML-SE211-0025	25 µg	168.00 USD

Ubiquitin-AMC is a fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). It is a particularly useful reagent for the study of deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (K_m = 0.039μM) (Prod. No. BML-UW9745) and for Isopeptidase-T (K_m = 0.17-1.4μM) (Prod. No. BML-UW9690), and is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.

Product Specification

Purity:	≥95% (HPLC)

Formulation:	In DMSO

Application:	1. Substrate for deubiquitinylating enzyme activity assays. 2. Identification/confirmation of enzyme deubiquitinylation activity. 3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates.

Long Term Storage:	-80°C

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	Please click here for the comprehensive product datasheet.

Figure: HPLC analysis. Comparison of Prod. No. BML-SE-211Prod. No. BML-SE-211) (A) with contaminated alternatively sourced material (B).

Figure: DUB Assay: 50nM UCLH1, His6-tagged (UW9740); 500nM Ubiquitin-AMC; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT.

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Product Literature References

Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry: D. E. Mason et al.; Biochemistry 43, 6535 (2004), Abstract;

Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes: L. C. Dang et al.; Biochemistry 37, 1868 (1868), Abstract;