Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Caspase-6 (human), (recombinant)

Highly, active caspase-6 for the study of enzyme kinetics or screening of inhibitors
 
BML-SE170-5000 5000 U 401.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Mch2
 
MW:18 + 11 kDa subunits
 
Source:Produced in E. coli.
 
UniProt ID:P55212
 
Concentration:100 U/µl
 
Formulation:Liquid. In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 0.5% CHAPS, 1mM EDTA, 10% glycerol and 10mM DTT.
 
Purity:≥80% (SDS-PAGE)
 
Specific Activity:One U=1 pmol/min at 30°C using Ac-VEID-pNA (200mM; Prod. No. BML-P437) as substrate.
 
Application Notes:Useful tool to study enzyme regulation and kinetics, cleave target substrates, screen for inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:Effector caspase responsible for the cleavage of nuclear lamins in apoptosis. Caspase-6 cleavages of various other protein targets have been implicated in the pathogenesis of neurodegenerative diseases, including Alzheimer’s disease (b-amyloid precursor protein), Huntington’s disease (huntingtin).
 
BML-SE170 SDS-PAGE
SDS-PAGE Analysis - Lane 1: MW Marker, Lane 2: 1 µg Caspase-6.
Please mouse over
BML-SE170 SDS-PAGE

Product Literature References

Laquinimod decreases Bax expression and reduces caspase-6 activation in neurons: D. Ehrnhoefer, et al.; Exp. Neurol. 283A, 121 (2016), Application(s): Caspase-6 inhibition (in vitro), Abstract;
Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant huntingtin protein but is not necessary for production of a 586 amino acid proteolytic fragment: J. Gafni, et al.; J. Neurosci 32, 7454 (2012), Abstract;
Induction of the C-terminal proteolytic cleavage of AβPP by statins: O. Descamps, et al.; J. Alzheimers Dis. 25, 51 (2011), Abstract;
Identification and Evaluation of Novel Small Molecule Pan-Caspase Inhibitors in Huntington’s Disease Models: M.J. Leyva, et al.; Chem. Biol. 17, 1189 (2010), Abstract;

General Literature References

Caspase-mediated cleavage of DNA topoisomerase I at unconventional sites during apoptosis: K. Samejima, et al.; J. Biol. Chem. 274, 4335 (1999), Abstract;
Biochemical characteristics of caspases-3, -6, -7, and -8: H.R. Stennicke & G.S. Salvesen; J. Biol. Chem. 272, 25719 (1997), Abstract;
Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells: L. Faleiro, et al.; EMBO J. 16, 2271 (1997), Abstract;
Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis: A. Takahashi, et al.; PNAS 93, 8395 (1996), Abstract;
The CED-3/ICE-like protease Mch2 is activated during apoptosis and cleaves the death substrate lamin A: K. Orth, et al.; J. Biol. Chem. 271, 16443 (1996), Abstract;
Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family: T. Fernandes-Alnemri, et al.; Cancer Res 55, 2737 (1995), Abstract;

Related Products

Caspase-6 (human), (recombinant) (active) 

Produced in E. coli. Contains an N-terminal His-tag., ≥95% (SDS-PAGE) | Print as PDF
 
ALX-201-060-U025 25 U 254.00 USD
 
ALX-201-060-U100 100 U 461.00 USD
Do you need bulk/larger quantities?
 

Z-VE(OMe)ID(OMe)-FMK 

Caspase-6 inhibitor
≥95% (TLC) | Print as PDF
 
ALX-260-075-M001 1 mg 265.00 USD
Do you need bulk/larger quantities?
 

Z-VEID-FMK (Ready-to-Use) 

Caspase-6 inhibitor
≥98% (HPLC) | Print as PDF
 
ALX-260-143-R100 100 µl 2mM 185.00 USD
 
ALX-260-143-R020 20 µl 10mM 185.00 USD
Do you need bulk/larger quantities?
 

Ac-VEID-AFC 

Caspase-6 inhibitor
≥98% (HPLC) | Print as PDF
 
ALX-260-111-M005 5 mg 175.00 USD
 
ALX-260-111-M010 10 mg 275.00 USD
Do you need bulk/larger quantities?
 

Ac-VEID-AMC 

Caspase-6 substrate
219137-97-0, ≥95% (HPLC) | Print as PDF
 
ALX-260-064-M001 1 mg 81.00 USD
 
ALX-260-064-M005 5 mg 139.00 USD
Do you need bulk/larger quantities?
 

Ac-VEID-pNA 

Caspase substrate
Synthetic., ≥95% (HPLC) | Print as PDF
 
BML-P437-0005 5 mg 118.00 USD
Do you need bulk/larger quantities?
 

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,