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Calcineurin (human), (recombinant)

 
BML-SE163-5000 5000 U 360.00 USD
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Product Specification

Alternative Name:Protein phosphatase 2B, PP2B
 
MW:60 kDa (calcineurin Aα) + 15 kDa (calcineurin B)
 
Source:Produced in E. coli. Human calcineurin Aα (MW 60kDa) and calcineurin B (MW 15kDa) heterodimer. Both subunits are coexpressed in a construct with yeast myristoyl-CoA:protein N-myristoyltransferase. The resulting highly active calcineurin (protein phosphatase 2B) is N-myristoylated on the CaNB subunit, similar to the native protein.
 
UniProt ID:Q08209 (subunit Aα), P63098 (subunit Bα)
 
Concentration:100 U/µl
 
Formulation:Liquid. In 1X KI-128 Assay Buffer with increased DTT concentration (50mM TRIS, pH 7.5, containing 100mM sodium chloride, 6mM MgCl2, 5mM DTT, 0.025% NP-40 and 0.5mM CaCl2).
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:One U= 1pmol/min using 0.15mM RII phosphopeptide substrate (Prod. No. BML-P160) in the presence of calcium/calmodulin at 30°C. In the absence of calmodulin, the activity is <1%.
 
Application Notes:Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates, and for screening inhibitors. Enzyme activity may be determined using the BIOMOL GREEN Calcineurin Assay Kit (Prod. No. BML-AK804).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
BML-SE163 SDS-PAGE
SDS-PAGE Analysis: Lane 1: MW Marker; Lane 2: 1.0 μg of purified human recombinant calcineurin protein (SE-163).
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BML-SE163 SDS-PAGE

Product Literature References

The phosphatase calcineurin regulates pathological TDP-43 phosphorylation: N.F. Liachko, et al.; Acta Neuropathol. 4, 545 (2016), Abstract;
Lysosomal calcium signalling regulates autophagy through calcineurin and ​TFEB: D.L. Medina, et al.; Nat. Cell. Biol. 17, 288 (2015), Application(s): Assay, Abstract;
Protein kinase C gamma-mediated phosphorylation of GluA1 in the postsynaptic density of spinal dorsal horn neurons accompanies neuropathic pain, and dephosphorylation by calcineurin is associated with prolonged analgesia: G. Miletic, et al.; Pain 156, 2514 (2015), Application(s): Intrathecal injection, Abstract; Full Text

General Literature References

Overexpression and purification of human calcineurin alpha from Escherichia coli and assessment of catalytic functions of residues surrounding the binuclear metal center: A. Mondragon, et al.; Biochemistry 34, 4934 (1997), Abstract;

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