Butyryl-lysine, pAb (affinity purified)



BML-SA682-0100	100 µl	272.00 USD

Product Specification

Formulation:	Liquid. Serum in PBS. The serum was immunodepleted to decrease reactivities with KLH, acetyl-lysine and propionyl-lysine.

Immunogen:	Butyrylated KLH.

Source/Host:	From rabbit.

Specificity:	Recognizes proteins containing butyrylated lysine. In Western blotting, minor cross-reactivity with propionyl-lysine can be minimized by adjustments to protein load and/or the antibody dilution (see Figure).

Application:	Western Blot (1:1’000-1:10’000) Optimal conditions must be determined individually for each application.

Long Term Storage:	-80°C

Handling:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Research demonstrates the occurrence of lysine post-translational acylations besides acetylation, including butyrylation. Lysine butyrylation has been observed in yeast and mammalian cells and on both histones and non-histone proteins. Acetyltransferases such as p300 and CBP have been found to catalyze butyrylation and deacetylases, notably the sirtuins, have been shown to have debutyrylation activity.

Background / Technical Information:	Please click here for the comprehensive product data sheet.

Figures: For each blot, the gel lane loadings were: 1) colored MW markers; 2) acetylated BSA; 3) propionylated BSA; 4) butyrylated BSA; 5) untreated BSA. In the gel for the upper blot loads in lanes 2-5 were 5 ng BSA, for the lower two blots, 50 ng BSA. Dilution of the anti-butyryl-lysine antibody was 1:1’000 for the upper and lower left blots and 1:10’000 for the lower right blot. Blots were incubated with the primary antibody overnight at 4°C, with the secondary (goat anti-rabbit-alkaline phosphatase) for 1.5 hr., at room temperature and developed with BCIP/NBT.

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General Literature References

Identification and verification of lysine propionylation and butyrylation in yeast core histones using PTMap software: K. Zhang, et al.; J. Proteome Res. 8, 900 (2009), Abstract;

Molecular characterization of propionyllysines in non-histone proteins: Z. Cheng, et al.; Mol. Cell. Proteomics 8, 45 (2009), Abstract;

Acetyl-lysine analog peptides as mechanistic probes of protein deacetylases: B.C. Smith & J.M. Denu; J. Biol. Chem. 282, 37256 (2007), Abstract;

Lysine propionylation and butyrylation are novel post-translational modifications in histones: Y. Chen, et al.; Mol. Cell. Proteomics 6, 812 (2007), Abstract;