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STIM1 (extracellular) polyclonal antibody

 
BML-SA676-0200 200 µl 470.00 USD
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Product Specification

Alternative Name:Stromal interaction molecule 1
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 56-69 (C56HSEDEKLSFEAVR69) of STIM1.
 
UniProt ID:Q13586
 
Species reactivity:Human, Rat
 
Applications:Flow Cytometry, ICC, IHC, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (5-10µg/5x105 cells)
Immunocytochemistry (1:20-1:100)
Western blot (1:500-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Blocking Peptide:40μg included. Lyophilized. Reconstitute with 100μl distilled water.
 
Purity Detail:Affinity purified.
 
Formulation:Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide.
 
Reconstitution:Reconstitute with 50μl or 200μl distilled water. Further dilutions should be made with medium containing a carrier protein such as 1% BSA.
 
Use/Stability:The antibody solution can be stored at +4°C for up to 2 weeks.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation. The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest to researchers. Several key players of the store operated complex have been identified; the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor3,4. The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.
 
Technical Info/Product Notes:Preadsorption control: Use 1µg peptide per 1µg antibody.  Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide.  Incubate at room temperature for 1 hr.  Centrifuge both tubes at 10,000Xg for 5 min.  Use the supernatants from tubes 1 and 2 for parallel experiments.
 
bml-sa676
Figure: Western blot analysis of RBL (lanes 1 & 4), HL-60 cell lysates (lanes 2 & 5) and Jurkat cell lysates (lanes 3 & 6).  Lanes 1, 2 and 3: PAb to STIM1 (Extracellular) (Prod. No. BML-SA676).  Lanes 4, 5 and 6: PAb to STIM1 (Extracellular) preincubated with the control.
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General Literature References

Dynamic coupling of the putative coiled-coil domain of ORAI1 with STIM1 mediates ORAI1 channel activation: M. Muik, et al.; J. Biol. Chem. 283, 8014 (2008), Abstract; Full Text
Biochemical properties and cellular localisation of STIM proteins: M.A. Dziadek & L.S Johnstone; Cell Calcium 42, 123 (2007), Abstract;
STIM1 heteromultimerizes TRPC channels to determine their function as store-operated channels: J.P. Yuan, et al.; Nat. Cell Biol. 9, 636 (2007), Abstract;
A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function: S. Feske, et al.; Nature 441, 179 (2006), Abstract;
Calcium signaling in non-excitable cells: Ca2+ release and influx are independent events linked to two plasma membrane Ca2+ entry channels: R. Chakrabarti & J. Chakrabarti; J. Cell. Biochem. 99, 1503 (2006), Abstract;
Calcium: You lose some, you gain some: J. Pickett; Nat. Rev. Mol. Cell Biol. 7, 794 (2006),
Stability and instability of regulation of intracellular calcium: D.A. Eisner, et al.; Exp. Physiol. 90, 3 (2005), Abstract; Full Text

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