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TRPM8 (extracellular) polyclonal antibody

 
BML-SA664-0050 50 µl 325.00 USD
 
BML-SA664-0200 200 µl 517.00 USD
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Product Specification

Alternative Name:Transient receptor potential channel subfamily M member 8
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 917-929 located in the third extracellular loop of human TRPM8.
 
UniProt ID:Q7Z2W7
 
Species reactivity:Human, Mouse, Rat
 
Applications:ICC, IP, WB
 
Blocking Peptide:Included: TRPM8 Control Peptide (40µg). Supplied lyophilized.
 
Purity Detail:Affinity purified IgG.
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
 
Reconstitution:To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. To lyophilized peptide, add 100µl of deionized water.
 
Use/Stability:Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas. The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family. TRPM8 is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.
 
Technical Info/Product Notes:PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
 
BML-SA664 WB
Western blot analysis of DU145 (lane 1 & 3) and LNCaP (lane 2 & 4) cells Anti-TRPM8 (1:200). Lanes 3 & 4: Anti-TRPM8 preincubated with the control peptide
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BML-SA664 WB

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